Conversion of carbon dioxide to oxaloacetate using integrated carbonic anhydrase and phosphoenolpyruvate carboxylase

Kwang Suk Chang, Hancheol Jeon, Man Bock Gu, Seung Pil Pack, Eonseon Jin

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The development and implementation of strategies for CO 2mitigation are necessary to counteract the greenhouse gas effect of carbon dioxide emissions. To demonstrate the possibility of simultaneously capturing CO2 and utilizing four-carbon compounds, an integrated system using CA and PEPCase was developed, which mimics an in vivo carbon dioxide concentration mechanism. We first cloned the PEPCase 1 gene of the marine diatom Phaeodactylum tricornutum and produced a recombinant PtPEPCase 1. The affinity column purified PtPEPCase 1 exhibited specific enzymatic activity (5.89 U/mg). When the simultaneous and coordinated reactions of CA from Dunaliella sp. and the PtPEPCase 1 occurred, more OAA was produced than when only PEPCase was present. Therefore, this integrated CA-PEPCase system can be used not only to capture CO2 but also for a new technology to produce value-added four-carbon platform chemicals.

Original languageEnglish
Pages (from-to)1923-1928
Number of pages6
JournalBioprocess and Biosystems Engineering
Volume36
Issue number12
DOIs
Publication statusPublished - 2013 Nov 1

Fingerprint

Phosphoenolpyruvate Carboxylase
Carbonic anhydrase
Oxaloacetic Acid
Carbonic Anhydrases
Carbon Dioxide
Carbon dioxide
Greenhouse Effect
Carbon
Diatoms
Carbon Monoxide
Greenhouse gases
Genes
Gases
Technology

Keywords

  • Bioconversion system
  • Carbonic anhydrase
  • Marine microalgae
  • Phosphoenolpyruvate carboxylase

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

Conversion of carbon dioxide to oxaloacetate using integrated carbonic anhydrase and phosphoenolpyruvate carboxylase. / Chang, Kwang Suk; Jeon, Hancheol; Gu, Man Bock; Pack, Seung Pil; Jin, Eonseon.

In: Bioprocess and Biosystems Engineering, Vol. 36, No. 12, 01.11.2013, p. 1923-1928.

Research output: Contribution to journalArticle

Chang, Kwang Suk ; Jeon, Hancheol ; Gu, Man Bock ; Pack, Seung Pil ; Jin, Eonseon. / Conversion of carbon dioxide to oxaloacetate using integrated carbonic anhydrase and phosphoenolpyruvate carboxylase. In: Bioprocess and Biosystems Engineering. 2013 ; Vol. 36, No. 12. pp. 1923-1928.
@article{80436c5852a54f419e9d19052d918c7b,
title = "Conversion of carbon dioxide to oxaloacetate using integrated carbonic anhydrase and phosphoenolpyruvate carboxylase",
abstract = "The development and implementation of strategies for CO 2mitigation are necessary to counteract the greenhouse gas effect of carbon dioxide emissions. To demonstrate the possibility of simultaneously capturing CO2 and utilizing four-carbon compounds, an integrated system using CA and PEPCase was developed, which mimics an in vivo carbon dioxide concentration mechanism. We first cloned the PEPCase 1 gene of the marine diatom Phaeodactylum tricornutum and produced a recombinant PtPEPCase 1. The affinity column purified PtPEPCase 1 exhibited specific enzymatic activity (5.89 U/mg). When the simultaneous and coordinated reactions of CA from Dunaliella sp. and the PtPEPCase 1 occurred, more OAA was produced than when only PEPCase was present. Therefore, this integrated CA-PEPCase system can be used not only to capture CO2 but also for a new technology to produce value-added four-carbon platform chemicals.",
keywords = "Bioconversion system, Carbonic anhydrase, Marine microalgae, Phosphoenolpyruvate carboxylase",
author = "Chang, {Kwang Suk} and Hancheol Jeon and Gu, {Man Bock} and Pack, {Seung Pil} and Eonseon Jin",
year = "2013",
month = "11",
day = "1",
doi = "10.1007/s00449-013-0968-5",
language = "English",
volume = "36",
pages = "1923--1928",
journal = "Bioprocess and Biosystems Engineering",
issn = "1615-7591",
publisher = "Springer Verlag",
number = "12",

}

TY - JOUR

T1 - Conversion of carbon dioxide to oxaloacetate using integrated carbonic anhydrase and phosphoenolpyruvate carboxylase

AU - Chang, Kwang Suk

AU - Jeon, Hancheol

AU - Gu, Man Bock

AU - Pack, Seung Pil

AU - Jin, Eonseon

PY - 2013/11/1

Y1 - 2013/11/1

N2 - The development and implementation of strategies for CO 2mitigation are necessary to counteract the greenhouse gas effect of carbon dioxide emissions. To demonstrate the possibility of simultaneously capturing CO2 and utilizing four-carbon compounds, an integrated system using CA and PEPCase was developed, which mimics an in vivo carbon dioxide concentration mechanism. We first cloned the PEPCase 1 gene of the marine diatom Phaeodactylum tricornutum and produced a recombinant PtPEPCase 1. The affinity column purified PtPEPCase 1 exhibited specific enzymatic activity (5.89 U/mg). When the simultaneous and coordinated reactions of CA from Dunaliella sp. and the PtPEPCase 1 occurred, more OAA was produced than when only PEPCase was present. Therefore, this integrated CA-PEPCase system can be used not only to capture CO2 but also for a new technology to produce value-added four-carbon platform chemicals.

AB - The development and implementation of strategies for CO 2mitigation are necessary to counteract the greenhouse gas effect of carbon dioxide emissions. To demonstrate the possibility of simultaneously capturing CO2 and utilizing four-carbon compounds, an integrated system using CA and PEPCase was developed, which mimics an in vivo carbon dioxide concentration mechanism. We first cloned the PEPCase 1 gene of the marine diatom Phaeodactylum tricornutum and produced a recombinant PtPEPCase 1. The affinity column purified PtPEPCase 1 exhibited specific enzymatic activity (5.89 U/mg). When the simultaneous and coordinated reactions of CA from Dunaliella sp. and the PtPEPCase 1 occurred, more OAA was produced than when only PEPCase was present. Therefore, this integrated CA-PEPCase system can be used not only to capture CO2 but also for a new technology to produce value-added four-carbon platform chemicals.

KW - Bioconversion system

KW - Carbonic anhydrase

KW - Marine microalgae

KW - Phosphoenolpyruvate carboxylase

UR - http://www.scopus.com/inward/record.url?scp=84892382719&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84892382719&partnerID=8YFLogxK

U2 - 10.1007/s00449-013-0968-5

DO - 10.1007/s00449-013-0968-5

M3 - Article

C2 - 23689757

AN - SCOPUS:84892382719

VL - 36

SP - 1923

EP - 1928

JO - Bioprocess and Biosystems Engineering

JF - Bioprocess and Biosystems Engineering

SN - 1615-7591

IS - 12

ER -