Conversion of carbon dioxide to oxaloacetate using integrated carbonic anhydrase and phosphoenolpyruvate carboxylase

Kwang Suk Chang, Hancheol Jeon, Man Bock Gu, Seung Pil Pack, Eonseon Jin

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The development and implementation of strategies for CO 2mitigation are necessary to counteract the greenhouse gas effect of carbon dioxide emissions. To demonstrate the possibility of simultaneously capturing CO2 and utilizing four-carbon compounds, an integrated system using CA and PEPCase was developed, which mimics an in vivo carbon dioxide concentration mechanism. We first cloned the PEPCase 1 gene of the marine diatom Phaeodactylum tricornutum and produced a recombinant PtPEPCase 1. The affinity column purified PtPEPCase 1 exhibited specific enzymatic activity (5.89 U/mg). When the simultaneous and coordinated reactions of CA from Dunaliella sp. and the PtPEPCase 1 occurred, more OAA was produced than when only PEPCase was present. Therefore, this integrated CA-PEPCase system can be used not only to capture CO2 but also for a new technology to produce value-added four-carbon platform chemicals.

Original languageEnglish
Pages (from-to)1923-1928
Number of pages6
JournalBioprocess and Biosystems Engineering
Volume36
Issue number12
DOIs
Publication statusPublished - 2013 Nov

Keywords

  • Bioconversion system
  • Carbonic anhydrase
  • Marine microalgae
  • Phosphoenolpyruvate carboxylase

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

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