Cooperative actions of Tra2α with 9G8 and SRp30c in the RNA splicing of the gonadotropin-releasing hormone gene transcript

Park Eonyoung, Han Jin, Gi Hoon Son, Sun Lee Mi, Chung Sooyoung, Ho Park Sung, Park Kyungsook, Ho Lee Kun, Choi Sukwoo, Jae Young Seong, Kim Kyungjin

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In earlier studies, we demonstrated that excision of the first intron (intron A) from the gonadotropin-releasing hormone (GnRH) transcript is highly cell type- and developmental stage-specific. The removal of GnRH intron A requires exonic splicing enhancers on exons 3 and 4 (ESE3 and ESE4, respectively). Tra2α, a serine/arginine-rich (SR)-like protein, specifically binds to ESE4, although it requires additional nuclear co-factors for efficient removal of this intron. In the present study, we demonstrate the cooperative action of multiple SR proteins in the regulation of GnRH pre-mRNA splicing. SRp30c specifically binds to both ESE3 and ESE4, whereas 9G8 binds to an element in exon 3 and strongly enhances the excision of GnRH intron A in the presence of minimal amount of other nuclear components. Interestingly, Tra2α can interact with either 9G8 or SRp30c, whereas no interaction between 9G8 and SRp30c is observed. Tra2α has an additive effect on the RNA binding of these proteins. Overexpression or knock-down of these three proteins in cultured cells further suggests their essential role in intron A excision activities, and their presence in GnRH neurons of the mouse preoptic area further strengthens this possibility. Together, these results indicate that interaction of Tra2α with 9G8 and SRp30c appears to be crucial for ESE-dependent GnRH pre-mRNA splicing, allowing efficient generation of mature mRNA in GnRH-producing cells.

Original languageEnglish
Pages (from-to)401-409
Number of pages9
JournalJournal of Biological Chemistry
Volume281
Issue number1
DOIs
Publication statusPublished - 2006 Jan 6

Fingerprint

RNA Splicing
Gonadotropin-Releasing Hormone
Introns
Genes
RNA
RNA Precursors
Serine
Arginine
Exons
Proteins
Preoptic Area
RNA-Binding Proteins
Neurons
Cultured Cells
Cells
Messenger RNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Cooperative actions of Tra2α with 9G8 and SRp30c in the RNA splicing of the gonadotropin-releasing hormone gene transcript. / Eonyoung, Park; Jin, Han; Son, Gi Hoon; Mi, Sun Lee; Sooyoung, Chung; Sung, Ho Park; Kyungsook, Park; Kun, Ho Lee; Sukwoo, Choi; Seong, Jae Young; Kyungjin, Kim.

In: Journal of Biological Chemistry, Vol. 281, No. 1, 06.01.2006, p. 401-409.

Research output: Contribution to journalArticle

Eonyoung, Park ; Jin, Han ; Son, Gi Hoon ; Mi, Sun Lee ; Sooyoung, Chung ; Sung, Ho Park ; Kyungsook, Park ; Kun, Ho Lee ; Sukwoo, Choi ; Seong, Jae Young ; Kyungjin, Kim. / Cooperative actions of Tra2α with 9G8 and SRp30c in the RNA splicing of the gonadotropin-releasing hormone gene transcript. In: Journal of Biological Chemistry. 2006 ; Vol. 281, No. 1. pp. 401-409.
@article{975d2568feae449e92e12f6c9c27470e,
title = "Cooperative actions of Tra2α with 9G8 and SRp30c in the RNA splicing of the gonadotropin-releasing hormone gene transcript",
abstract = "In earlier studies, we demonstrated that excision of the first intron (intron A) from the gonadotropin-releasing hormone (GnRH) transcript is highly cell type- and developmental stage-specific. The removal of GnRH intron A requires exonic splicing enhancers on exons 3 and 4 (ESE3 and ESE4, respectively). Tra2α, a serine/arginine-rich (SR)-like protein, specifically binds to ESE4, although it requires additional nuclear co-factors for efficient removal of this intron. In the present study, we demonstrate the cooperative action of multiple SR proteins in the regulation of GnRH pre-mRNA splicing. SRp30c specifically binds to both ESE3 and ESE4, whereas 9G8 binds to an element in exon 3 and strongly enhances the excision of GnRH intron A in the presence of minimal amount of other nuclear components. Interestingly, Tra2α can interact with either 9G8 or SRp30c, whereas no interaction between 9G8 and SRp30c is observed. Tra2α has an additive effect on the RNA binding of these proteins. Overexpression or knock-down of these three proteins in cultured cells further suggests their essential role in intron A excision activities, and their presence in GnRH neurons of the mouse preoptic area further strengthens this possibility. Together, these results indicate that interaction of Tra2α with 9G8 and SRp30c appears to be crucial for ESE-dependent GnRH pre-mRNA splicing, allowing efficient generation of mature mRNA in GnRH-producing cells.",
author = "Park Eonyoung and Han Jin and Son, {Gi Hoon} and Mi, {Sun Lee} and Chung Sooyoung and Sung, {Ho Park} and Park Kyungsook and Kun, {Ho Lee} and Choi Sukwoo and Seong, {Jae Young} and Kim Kyungjin",
year = "2006",
month = "1",
day = "6",
doi = "10.1074/jbc.M505814200",
language = "English",
volume = "281",
pages = "401--409",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "1",

}

TY - JOUR

T1 - Cooperative actions of Tra2α with 9G8 and SRp30c in the RNA splicing of the gonadotropin-releasing hormone gene transcript

AU - Eonyoung, Park

AU - Jin, Han

AU - Son, Gi Hoon

AU - Mi, Sun Lee

AU - Sooyoung, Chung

AU - Sung, Ho Park

AU - Kyungsook, Park

AU - Kun, Ho Lee

AU - Sukwoo, Choi

AU - Seong, Jae Young

AU - Kyungjin, Kim

PY - 2006/1/6

Y1 - 2006/1/6

N2 - In earlier studies, we demonstrated that excision of the first intron (intron A) from the gonadotropin-releasing hormone (GnRH) transcript is highly cell type- and developmental stage-specific. The removal of GnRH intron A requires exonic splicing enhancers on exons 3 and 4 (ESE3 and ESE4, respectively). Tra2α, a serine/arginine-rich (SR)-like protein, specifically binds to ESE4, although it requires additional nuclear co-factors for efficient removal of this intron. In the present study, we demonstrate the cooperative action of multiple SR proteins in the regulation of GnRH pre-mRNA splicing. SRp30c specifically binds to both ESE3 and ESE4, whereas 9G8 binds to an element in exon 3 and strongly enhances the excision of GnRH intron A in the presence of minimal amount of other nuclear components. Interestingly, Tra2α can interact with either 9G8 or SRp30c, whereas no interaction between 9G8 and SRp30c is observed. Tra2α has an additive effect on the RNA binding of these proteins. Overexpression or knock-down of these three proteins in cultured cells further suggests their essential role in intron A excision activities, and their presence in GnRH neurons of the mouse preoptic area further strengthens this possibility. Together, these results indicate that interaction of Tra2α with 9G8 and SRp30c appears to be crucial for ESE-dependent GnRH pre-mRNA splicing, allowing efficient generation of mature mRNA in GnRH-producing cells.

AB - In earlier studies, we demonstrated that excision of the first intron (intron A) from the gonadotropin-releasing hormone (GnRH) transcript is highly cell type- and developmental stage-specific. The removal of GnRH intron A requires exonic splicing enhancers on exons 3 and 4 (ESE3 and ESE4, respectively). Tra2α, a serine/arginine-rich (SR)-like protein, specifically binds to ESE4, although it requires additional nuclear co-factors for efficient removal of this intron. In the present study, we demonstrate the cooperative action of multiple SR proteins in the regulation of GnRH pre-mRNA splicing. SRp30c specifically binds to both ESE3 and ESE4, whereas 9G8 binds to an element in exon 3 and strongly enhances the excision of GnRH intron A in the presence of minimal amount of other nuclear components. Interestingly, Tra2α can interact with either 9G8 or SRp30c, whereas no interaction between 9G8 and SRp30c is observed. Tra2α has an additive effect on the RNA binding of these proteins. Overexpression or knock-down of these three proteins in cultured cells further suggests their essential role in intron A excision activities, and their presence in GnRH neurons of the mouse preoptic area further strengthens this possibility. Together, these results indicate that interaction of Tra2α with 9G8 and SRp30c appears to be crucial for ESE-dependent GnRH pre-mRNA splicing, allowing efficient generation of mature mRNA in GnRH-producing cells.

UR - http://www.scopus.com/inward/record.url?scp=33644780370&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33644780370&partnerID=8YFLogxK

U2 - 10.1074/jbc.M505814200

DO - 10.1074/jbc.M505814200

M3 - Article

VL - 281

SP - 401

EP - 409

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 1

ER -