Copine1 C2 domains have a critical calcium-independent role in the neuronal differentiation of hippocampal progenitor HiB5 cells

Nammi Park, Jae Cheal Yoo, Young Sun Lee, Hye Young Choi, Seong Geun Hong, Eun Mi Hwang, Jae-Yong Park

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Copine1 (CPNE1) has tandem C2 domains and an A domain and is known as a calcium-dependent membrane-binding protein that regulates signal transduction and membrane trafficking. We previously demonstrated that CPNE1 directly induces neuronal differentiation via Akt phosphorylation in the hippocampal progenitor cell line, HiB5. To determine which region of CPNE1 is related to HiB5 cell neurite outgrowth, we constructed several mutants. Our results show that over-expression of each C2 domain of CPNE1 increased neurite outgrowth and expression of the neuronal marker protein neurofilament (NF). Even though protein localization of the calcium binding-deficient mutant of CPNE1 was not affected by ionomycin, this mutant increased neurite outgrowth and NF expression in HiB5 cells. Furthermore, Akt phosphorylation was increased by over-expression of the calcium binding-deficient CPNE1 mutant. These results suggest that neither cellular calcium levels nor the localization of CPNE1 affect its function in neuronal differentiation. Collectively, our findings indicating that the C2 domains of CPNE1 play a calcium-independent role in regulating the neuronal differentiation of HiB5 cells.

Original languageEnglish
Pages (from-to)228-233
Number of pages6
JournalBiochemical and biophysical research communications
Volume454
Issue number1
DOIs
Publication statusPublished - 2014 Nov 7

Fingerprint

Stem Cells
Calcium
Phosphorylation
Neurofilament Proteins
Ionomycin
Calcium-Binding Proteins
Intermediate Filaments
Membranes
Signal transduction
Cell Differentiation
Signal Transduction
Carrier Proteins
Membrane Proteins
Cell Line
Cells
C2 Domains
Neuronal Outgrowth
Proteins

Keywords

  • Akt
  • C2 domain
  • Copine1
  • HiB5 cell
  • Neuronal differentiation
  • NF

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Copine1 C2 domains have a critical calcium-independent role in the neuronal differentiation of hippocampal progenitor HiB5 cells. / Park, Nammi; Yoo, Jae Cheal; Lee, Young Sun; Choi, Hye Young; Hong, Seong Geun; Hwang, Eun Mi; Park, Jae-Yong.

In: Biochemical and biophysical research communications, Vol. 454, No. 1, 07.11.2014, p. 228-233.

Research output: Contribution to journalArticle

Park, Nammi ; Yoo, Jae Cheal ; Lee, Young Sun ; Choi, Hye Young ; Hong, Seong Geun ; Hwang, Eun Mi ; Park, Jae-Yong. / Copine1 C2 domains have a critical calcium-independent role in the neuronal differentiation of hippocampal progenitor HiB5 cells. In: Biochemical and biophysical research communications. 2014 ; Vol. 454, No. 1. pp. 228-233.
@article{4f7ea77931714e2191b090127c43b98f,
title = "Copine1 C2 domains have a critical calcium-independent role in the neuronal differentiation of hippocampal progenitor HiB5 cells",
abstract = "Copine1 (CPNE1) has tandem C2 domains and an A domain and is known as a calcium-dependent membrane-binding protein that regulates signal transduction and membrane trafficking. We previously demonstrated that CPNE1 directly induces neuronal differentiation via Akt phosphorylation in the hippocampal progenitor cell line, HiB5. To determine which region of CPNE1 is related to HiB5 cell neurite outgrowth, we constructed several mutants. Our results show that over-expression of each C2 domain of CPNE1 increased neurite outgrowth and expression of the neuronal marker protein neurofilament (NF). Even though protein localization of the calcium binding-deficient mutant of CPNE1 was not affected by ionomycin, this mutant increased neurite outgrowth and NF expression in HiB5 cells. Furthermore, Akt phosphorylation was increased by over-expression of the calcium binding-deficient CPNE1 mutant. These results suggest that neither cellular calcium levels nor the localization of CPNE1 affect its function in neuronal differentiation. Collectively, our findings indicating that the C2 domains of CPNE1 play a calcium-independent role in regulating the neuronal differentiation of HiB5 cells.",
keywords = "Akt, C2 domain, Copine1, HiB5 cell, Neuronal differentiation, NF",
author = "Nammi Park and Yoo, {Jae Cheal} and Lee, {Young Sun} and Choi, {Hye Young} and Hong, {Seong Geun} and Hwang, {Eun Mi} and Jae-Yong Park",
year = "2014",
month = "11",
day = "7",
doi = "10.1016/j.bbrc.2014.10.075",
language = "English",
volume = "454",
pages = "228--233",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Copine1 C2 domains have a critical calcium-independent role in the neuronal differentiation of hippocampal progenitor HiB5 cells

AU - Park, Nammi

AU - Yoo, Jae Cheal

AU - Lee, Young Sun

AU - Choi, Hye Young

AU - Hong, Seong Geun

AU - Hwang, Eun Mi

AU - Park, Jae-Yong

PY - 2014/11/7

Y1 - 2014/11/7

N2 - Copine1 (CPNE1) has tandem C2 domains and an A domain and is known as a calcium-dependent membrane-binding protein that regulates signal transduction and membrane trafficking. We previously demonstrated that CPNE1 directly induces neuronal differentiation via Akt phosphorylation in the hippocampal progenitor cell line, HiB5. To determine which region of CPNE1 is related to HiB5 cell neurite outgrowth, we constructed several mutants. Our results show that over-expression of each C2 domain of CPNE1 increased neurite outgrowth and expression of the neuronal marker protein neurofilament (NF). Even though protein localization of the calcium binding-deficient mutant of CPNE1 was not affected by ionomycin, this mutant increased neurite outgrowth and NF expression in HiB5 cells. Furthermore, Akt phosphorylation was increased by over-expression of the calcium binding-deficient CPNE1 mutant. These results suggest that neither cellular calcium levels nor the localization of CPNE1 affect its function in neuronal differentiation. Collectively, our findings indicating that the C2 domains of CPNE1 play a calcium-independent role in regulating the neuronal differentiation of HiB5 cells.

AB - Copine1 (CPNE1) has tandem C2 domains and an A domain and is known as a calcium-dependent membrane-binding protein that regulates signal transduction and membrane trafficking. We previously demonstrated that CPNE1 directly induces neuronal differentiation via Akt phosphorylation in the hippocampal progenitor cell line, HiB5. To determine which region of CPNE1 is related to HiB5 cell neurite outgrowth, we constructed several mutants. Our results show that over-expression of each C2 domain of CPNE1 increased neurite outgrowth and expression of the neuronal marker protein neurofilament (NF). Even though protein localization of the calcium binding-deficient mutant of CPNE1 was not affected by ionomycin, this mutant increased neurite outgrowth and NF expression in HiB5 cells. Furthermore, Akt phosphorylation was increased by over-expression of the calcium binding-deficient CPNE1 mutant. These results suggest that neither cellular calcium levels nor the localization of CPNE1 affect its function in neuronal differentiation. Collectively, our findings indicating that the C2 domains of CPNE1 play a calcium-independent role in regulating the neuronal differentiation of HiB5 cells.

KW - Akt

KW - C2 domain

KW - Copine1

KW - HiB5 cell

KW - Neuronal differentiation

KW - NF

UR - http://www.scopus.com/inward/record.url?scp=84910634240&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84910634240&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2014.10.075

DO - 10.1016/j.bbrc.2014.10.075

M3 - Article

VL - 454

SP - 228

EP - 233

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -