Copine1 regulates neural stem cell functions during brain development

Tae Hwan Kim, Soo Eun Sung, Jae Cheal Yoo, Jae-Yong Park, Gwan su Yi, Jun Young Heo, Jae Ran Lee, Nam Soon Kim, Da Yong Lee

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Copine 1 (CPNE1) is a well-known phospholipid binding protein in plasma membrane of various cell types. In brain cells, CPNE1 is closely associated with AKT signaling pathway, which is important for neural stem cell (NSC) functions during brain development. Here, we investigated the role of CPNE1 in the regulation of brain NSC functions during brain development and determined its underlying mechanism. In this study, abundant expression of CPNE1 was observed in neural lineage cells including NSCs and immature neurons in human. With mouse brain tissues in various developmental stages, we found that CPNE1 expression was higher at early embryonic stages compared to postnatal and adult stages. To model developing brain in vitro, we used primary NSCs derived from mouse embryonic hippocampus. Our in vitro study shows decreased proliferation and multi-lineage differentiation potential in CPNE1 deficient NSCs. Finally, we found that the deficiency of CPNE1 downregulated mTOR signaling in embryonic NSCs. These data demonstrate that CPNE1 plays a key role in the regulation of NSC functions through the activation of AKT-mTOR signaling pathway during brain development.

Original languageEnglish
Pages (from-to)168-173
Number of pages6
JournalBiochemical and biophysical research communications
Volume495
Issue number1
DOIs
Publication statusPublished - 2018 Jan 1

Fingerprint

Neural Stem Cells
Stem cells
Brain
copine
Cell membranes
Brain Stem
Neurons
Hippocampus
Phospholipids
Carrier Proteins
Down-Regulation
Chemical activation
Cell Membrane
Tissue

Keywords

  • Copine1
  • Gliogenesis
  • mTOR
  • Neural stem cell
  • Neurogenesis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Copine1 regulates neural stem cell functions during brain development. / Kim, Tae Hwan; Sung, Soo Eun; Cheal Yoo, Jae; Park, Jae-Yong; Yi, Gwan su; Heo, Jun Young; Lee, Jae Ran; Kim, Nam Soon; Lee, Da Yong.

In: Biochemical and biophysical research communications, Vol. 495, No. 1, 01.01.2018, p. 168-173.

Research output: Contribution to journalArticle

Kim, TH, Sung, SE, Cheal Yoo, J, Park, J-Y, Yi, GS, Heo, JY, Lee, JR, Kim, NS & Lee, DY 2018, 'Copine1 regulates neural stem cell functions during brain development', Biochemical and biophysical research communications, vol. 495, no. 1, pp. 168-173. https://doi.org/10.1016/j.bbrc.2017.10.167
Kim, Tae Hwan ; Sung, Soo Eun ; Cheal Yoo, Jae ; Park, Jae-Yong ; Yi, Gwan su ; Heo, Jun Young ; Lee, Jae Ran ; Kim, Nam Soon ; Lee, Da Yong. / Copine1 regulates neural stem cell functions during brain development. In: Biochemical and biophysical research communications. 2018 ; Vol. 495, No. 1. pp. 168-173.
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