Correlation between in vitro maturation and expression of LH receptor in cumulus cells of the oocytes collected from PCOS patients in HCG-primed IVM cycles

Seong H. Yang, Weon Young Son, San Hyun Yoon, Yong Ko, Jin H. Lim

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Background: The aim of this study was to investigate whether in vitro maturation (IVM) and blastocyst development of oocytes collected following HCG-primed IVM cycles of PCOS patients are correlated with their cumulus cell (CC) patterns and further to investigate mRNA expression of the receptors for FSH, LH and epidermal growth factor (EGF) in the CCs with each pattern. Methods: Patients who underwent IVM were primed with 10 000 IU of HCG 36 h before oocyte aspiration. The isolated cumulus-oocyte complexes were divided into three groups according to the CC patterns: oocytes with dispersed CCs (group A), oocytes with compacted CCs (group B) and oocytes with sparse CCs (group C). Oocyte maturation and blastocyst development were compared among three groups. The expression of the mRNA for FSH, LH and EGF receptors in group A and B was analysed by semi-quantitative RT-PCR. Results: The maturation rate of group A was significantly higher than those of group B and C. The rate of blastocysts in group A was significantly higher than those of group B and C. mRNA expression of the LH reeptor in group A was more abundant than that of group B. Conclusions: These results suggest that the presence of dispersed CCs at oocyte collection may be positively correlated with the rates of oocyte maturation and blastocysts in HCG-primed IVM cycles. In addition, the expression of LH receptor in CCs may be correlated with the CC pattern of oocytes at collection.

Original languageEnglish
Pages (from-to)2097-2103
Number of pages7
JournalHuman Reproduction
Volume20
Issue number8
DOIs
Publication statusPublished - 2005 Aug 1

Fingerprint

Cumulus Cells
LH Receptors
Oocytes
Blastocyst
Oocyte Retrieval
Messenger RNA
FSH Receptors
In Vitro Techniques
Epidermal Growth Factor
Polymerase Chain Reaction

Keywords

  • Cumulus cell pattern
  • HCG
  • Human
  • Immature oocytes
  • IVM

ASJC Scopus subject areas

  • Developmental Biology
  • Physiology
  • Obstetrics and Gynaecology
  • Reproductive Medicine

Cite this

Correlation between in vitro maturation and expression of LH receptor in cumulus cells of the oocytes collected from PCOS patients in HCG-primed IVM cycles. / Yang, Seong H.; Son, Weon Young; Yoon, San Hyun; Ko, Yong; Lim, Jin H.

In: Human Reproduction, Vol. 20, No. 8, 01.08.2005, p. 2097-2103.

Research output: Contribution to journalArticle

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