Correlation between specific IgA and eosinophil numbers in the lavage fluid of patients with perennial allergic rhinitis

Jeong Hoon Oh, Gyu Young Hur, Young Min Ye, Jeong Eun Kim, Keehyun Park, Hae Sim Park

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The mechanisms of eosinophil activation in perennial allergic rhinitis (AR) are not fully understood but may be mediated by a series of inflammatory mediators, including locally produced specific antibodies. The aim of this study was to evaluate the role of locally produced allergen-specific antibodies in the nasal lavage fluid during the recruitment of inflammatory cells in AR. Thirteen patients with AR and a positive nasal provocation test (NPT) with Dermatophagoides pteronyssinus and 10 asymptomatic subjects, sensitized to D. pteronyssinus but who had a negative NPT were enrolled. Nasal lavage fluids were collected before and at 10, 30, and 60 minutes and 3, 6, and 24 hours after the NPT. Inflammatory cells were counted, and eosinophil cationic protein (ECP) and specific immunoglobulin E (IgE), IgG, and IgA antibodies were measured by enzyme-linked immunosorbent assay. After NPT, six subjects showed early responses and seven subjects showed dual responses. The eosinophil count and ECP level in lavage fluid were significantly increased after NPT in patients with positive NPTs, and these values were significantly higher than in the negative group. The specific IgA level in lavage fluid was increased after NPT in the positive group, and significant differences were noted for both the early and the late positive responses. Significant correlations were found between specific IgA and ECP levels in lavage fluid during early and late responses. Eosinophil recruitment occurred during both early and late responses after allergen exposure in perennial AR. Locally produced allergen-specific IgA (but not IgE or IgG) may be involved in eosinophil activation in both early and late responses.

Original languageEnglish
Pages (from-to)152-160
Number of pages9
JournalAllergy and Asthma Proceedings
Volume29
Issue number2
DOIs
Publication statusPublished - 2008 Mar 1
Externally publishedYes

Fingerprint

Nasal Provocation Tests
Rhinitis, Allergic, Perennial
Therapeutic Irrigation
Eosinophils
Immunoglobulin A
Eosinophil Cationic Protein
Nasal Lavage Fluid
Allergens
Dermatophagoides pteronyssinus
Immunoglobulin E
Antibodies
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay

Keywords

  • Antigen challenge
  • ECP
  • Eosinophil recruitment
  • IgA
  • IgE
  • IgG
  • Late phase
  • Nasal provocation
  • Perennial allergic rhinitis
  • Specific antibody

ASJC Scopus subject areas

  • Immunology and Allergy
  • Pulmonary and Respiratory Medicine

Cite this

Correlation between specific IgA and eosinophil numbers in the lavage fluid of patients with perennial allergic rhinitis. / Oh, Jeong Hoon; Hur, Gyu Young; Ye, Young Min; Kim, Jeong Eun; Park, Keehyun; Park, Hae Sim.

In: Allergy and Asthma Proceedings, Vol. 29, No. 2, 01.03.2008, p. 152-160.

Research output: Contribution to journalArticle

Oh, Jeong Hoon ; Hur, Gyu Young ; Ye, Young Min ; Kim, Jeong Eun ; Park, Keehyun ; Park, Hae Sim. / Correlation between specific IgA and eosinophil numbers in the lavage fluid of patients with perennial allergic rhinitis. In: Allergy and Asthma Proceedings. 2008 ; Vol. 29, No. 2. pp. 152-160.
@article{b7bf31dfe20b4d1e922aab4e4446ddb7,
title = "Correlation between specific IgA and eosinophil numbers in the lavage fluid of patients with perennial allergic rhinitis",
abstract = "The mechanisms of eosinophil activation in perennial allergic rhinitis (AR) are not fully understood but may be mediated by a series of inflammatory mediators, including locally produced specific antibodies. The aim of this study was to evaluate the role of locally produced allergen-specific antibodies in the nasal lavage fluid during the recruitment of inflammatory cells in AR. Thirteen patients with AR and a positive nasal provocation test (NPT) with Dermatophagoides pteronyssinus and 10 asymptomatic subjects, sensitized to D. pteronyssinus but who had a negative NPT were enrolled. Nasal lavage fluids were collected before and at 10, 30, and 60 minutes and 3, 6, and 24 hours after the NPT. Inflammatory cells were counted, and eosinophil cationic protein (ECP) and specific immunoglobulin E (IgE), IgG, and IgA antibodies were measured by enzyme-linked immunosorbent assay. After NPT, six subjects showed early responses and seven subjects showed dual responses. The eosinophil count and ECP level in lavage fluid were significantly increased after NPT in patients with positive NPTs, and these values were significantly higher than in the negative group. The specific IgA level in lavage fluid was increased after NPT in the positive group, and significant differences were noted for both the early and the late positive responses. Significant correlations were found between specific IgA and ECP levels in lavage fluid during early and late responses. Eosinophil recruitment occurred during both early and late responses after allergen exposure in perennial AR. Locally produced allergen-specific IgA (but not IgE or IgG) may be involved in eosinophil activation in both early and late responses.",
keywords = "Antigen challenge, ECP, Eosinophil recruitment, IgA, IgE, IgG, Late phase, Nasal provocation, Perennial allergic rhinitis, Specific antibody",
author = "Oh, {Jeong Hoon} and Hur, {Gyu Young} and Ye, {Young Min} and Kim, {Jeong Eun} and Keehyun Park and Park, {Hae Sim}",
year = "2008",
month = "3",
day = "1",
doi = "10.2500/aap.2008.29.3069",
language = "English",
volume = "29",
pages = "152--160",
journal = "Allergy and Asthma Proceedings",
issn = "1088-5412",
publisher = "OceanSide Publications Inc.",
number = "2",

}

TY - JOUR

T1 - Correlation between specific IgA and eosinophil numbers in the lavage fluid of patients with perennial allergic rhinitis

AU - Oh, Jeong Hoon

AU - Hur, Gyu Young

AU - Ye, Young Min

AU - Kim, Jeong Eun

AU - Park, Keehyun

AU - Park, Hae Sim

PY - 2008/3/1

Y1 - 2008/3/1

N2 - The mechanisms of eosinophil activation in perennial allergic rhinitis (AR) are not fully understood but may be mediated by a series of inflammatory mediators, including locally produced specific antibodies. The aim of this study was to evaluate the role of locally produced allergen-specific antibodies in the nasal lavage fluid during the recruitment of inflammatory cells in AR. Thirteen patients with AR and a positive nasal provocation test (NPT) with Dermatophagoides pteronyssinus and 10 asymptomatic subjects, sensitized to D. pteronyssinus but who had a negative NPT were enrolled. Nasal lavage fluids were collected before and at 10, 30, and 60 minutes and 3, 6, and 24 hours after the NPT. Inflammatory cells were counted, and eosinophil cationic protein (ECP) and specific immunoglobulin E (IgE), IgG, and IgA antibodies were measured by enzyme-linked immunosorbent assay. After NPT, six subjects showed early responses and seven subjects showed dual responses. The eosinophil count and ECP level in lavage fluid were significantly increased after NPT in patients with positive NPTs, and these values were significantly higher than in the negative group. The specific IgA level in lavage fluid was increased after NPT in the positive group, and significant differences were noted for both the early and the late positive responses. Significant correlations were found between specific IgA and ECP levels in lavage fluid during early and late responses. Eosinophil recruitment occurred during both early and late responses after allergen exposure in perennial AR. Locally produced allergen-specific IgA (but not IgE or IgG) may be involved in eosinophil activation in both early and late responses.

AB - The mechanisms of eosinophil activation in perennial allergic rhinitis (AR) are not fully understood but may be mediated by a series of inflammatory mediators, including locally produced specific antibodies. The aim of this study was to evaluate the role of locally produced allergen-specific antibodies in the nasal lavage fluid during the recruitment of inflammatory cells in AR. Thirteen patients with AR and a positive nasal provocation test (NPT) with Dermatophagoides pteronyssinus and 10 asymptomatic subjects, sensitized to D. pteronyssinus but who had a negative NPT were enrolled. Nasal lavage fluids were collected before and at 10, 30, and 60 minutes and 3, 6, and 24 hours after the NPT. Inflammatory cells were counted, and eosinophil cationic protein (ECP) and specific immunoglobulin E (IgE), IgG, and IgA antibodies were measured by enzyme-linked immunosorbent assay. After NPT, six subjects showed early responses and seven subjects showed dual responses. The eosinophil count and ECP level in lavage fluid were significantly increased after NPT in patients with positive NPTs, and these values were significantly higher than in the negative group. The specific IgA level in lavage fluid was increased after NPT in the positive group, and significant differences were noted for both the early and the late positive responses. Significant correlations were found between specific IgA and ECP levels in lavage fluid during early and late responses. Eosinophil recruitment occurred during both early and late responses after allergen exposure in perennial AR. Locally produced allergen-specific IgA (but not IgE or IgG) may be involved in eosinophil activation in both early and late responses.

KW - Antigen challenge

KW - ECP

KW - Eosinophil recruitment

KW - IgA

KW - IgE

KW - IgG

KW - Late phase

KW - Nasal provocation

KW - Perennial allergic rhinitis

KW - Specific antibody

UR - http://www.scopus.com/inward/record.url?scp=43149091465&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=43149091465&partnerID=8YFLogxK

U2 - 10.2500/aap.2008.29.3069

DO - 10.2500/aap.2008.29.3069

M3 - Article

VL - 29

SP - 152

EP - 160

JO - Allergy and Asthma Proceedings

JF - Allergy and Asthma Proceedings

SN - 1088-5412

IS - 2

ER -