Abstract
Several in vitro chemosensitivity tests have been developed to predict the chemotherapeutic response of tumours prior to initiation of individualized treatment for breast cancer. This study investigated whether the in vitro chemosensitivity response of cell lines derived from breast cancer patients was affected by HER2/neu expression. We cultured breast cancer cell lines from 50 patients and the adenosine triphosphate-based chemotherapy response assay (ATP-CRA) was performed with 5-fluorouracil, gemcitabine, docetaxel, doxorubicin, methotrexate, vinorelbine and paclitaxel. cell death rate (32.4%) with the narrowest range of cytotoxic effects (7.3 - 65.7%). In addition, gemcitabine showed significantly greater activity in HER2/neu-positive patients. In contrast, docetaxel was significantly less effective in HER2/ neu-positive patients. No significant correlation was found between the other agents and HER2/neu expression. The use of the ATP-CRA test for metastatic tissue from patients with recurrent disease might be a useful approach to determine the most effective chemotherapy regimen.
| Original language | English |
|---|---|
| Pages (from-to) | 753-761 |
| Number of pages | 9 |
| Journal | Journal of International Medical Research |
| Volume | 35 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2007 Jan 1 |
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Keywords
- 5-Flourouracil
- Anticancer Agents
- Breast cancer
- Chemosensitivity
- Doxorubicin
- HER2/neu
- Hormone receptor
- Methotrexate
- Vinorelbine
ASJC Scopus subject areas
- Biochemistry
- Cell Biology
- Biochemistry, medical
Cite this
Correlation between the in vitro ATP-based chemosensitivity assay and HER2/neu expression in women with breast cancer. / Woo, Sang- Uk; Bae, Jeoug Won; Kim, H. G.; Choi, S. H.; Kang, D. H.; Lee, Jae Bok; Koo, B. W.
In: Journal of International Medical Research, Vol. 35, No. 6, 01.01.2007, p. 753-761.Research output: Contribution to journal › Review article
}
TY - JOUR
T1 - Correlation between the in vitro ATP-based chemosensitivity assay and HER2/neu expression in women with breast cancer
AU - Woo, Sang- Uk
AU - Bae, Jeoug Won
AU - Kim, H. G.
AU - Choi, S. H.
AU - Kang, D. H.
AU - Lee, Jae Bok
AU - Koo, B. W.
PY - 2007/1/1
Y1 - 2007/1/1
N2 - Several in vitro chemosensitivity tests have been developed to predict the chemotherapeutic response of tumours prior to initiation of individualized treatment for breast cancer. This study investigated whether the in vitro chemosensitivity response of cell lines derived from breast cancer patients was affected by HER2/neu expression. We cultured breast cancer cell lines from 50 patients and the adenosine triphosphate-based chemotherapy response assay (ATP-CRA) was performed with 5-fluorouracil, gemcitabine, docetaxel, doxorubicin, methotrexate, vinorelbine and paclitaxel. cell death rate (32.4%) with the narrowest range of cytotoxic effects (7.3 - 65.7%). In addition, gemcitabine showed significantly greater activity in HER2/neu-positive patients. In contrast, docetaxel was significantly less effective in HER2/ neu-positive patients. No significant correlation was found between the other agents and HER2/neu expression. The use of the ATP-CRA test for metastatic tissue from patients with recurrent disease might be a useful approach to determine the most effective chemotherapy regimen.
AB - Several in vitro chemosensitivity tests have been developed to predict the chemotherapeutic response of tumours prior to initiation of individualized treatment for breast cancer. This study investigated whether the in vitro chemosensitivity response of cell lines derived from breast cancer patients was affected by HER2/neu expression. We cultured breast cancer cell lines from 50 patients and the adenosine triphosphate-based chemotherapy response assay (ATP-CRA) was performed with 5-fluorouracil, gemcitabine, docetaxel, doxorubicin, methotrexate, vinorelbine and paclitaxel. cell death rate (32.4%) with the narrowest range of cytotoxic effects (7.3 - 65.7%). In addition, gemcitabine showed significantly greater activity in HER2/neu-positive patients. In contrast, docetaxel was significantly less effective in HER2/ neu-positive patients. No significant correlation was found between the other agents and HER2/neu expression. The use of the ATP-CRA test for metastatic tissue from patients with recurrent disease might be a useful approach to determine the most effective chemotherapy regimen.
KW - 5-Flourouracil
KW - Anticancer Agents
KW - Breast cancer
KW - Chemosensitivity
KW - Doxorubicin
KW - HER2/neu
KW - Hormone receptor
KW - Methotrexate
KW - Vinorelbine
UR - http://www.scopus.com/inward/record.url?scp=36949033840&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=36949033840&partnerID=8YFLogxK
U2 - 10.1177/147323000703500603
DO - 10.1177/147323000703500603
M3 - Review article
VL - 35
SP - 753
EP - 761
JO - Journal of International Medical Research
JF - Journal of International Medical Research
SN - 0300-0605
IS - 6
ER -