Correlative super-resolution fluorescence microscopy combined with optical coherence microscopy

Sungho Kim, Gyeong Tae Kim, Soohyun Jang, Sang-Hee Shim, Sung Chul Bae

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Recent development of super-resolution fluorescence imaging technique such as stochastic optical reconstruction microscopy (STORM) and photoactived localization microscope (PALM) has brought us beyond the diffraction limits. It allows numerous opportunities in biology because vast amount of formerly obscured molecular structures, due to lack of spatial resolution, now can be directly observed. A drawback of fluorescence imaging, however, is that it lacks complete structural information. For this reason, we have developed a super-resolution multimodal imaging system based on STORM and full-field optical coherence microscopy (FF-OCM). FF-OCM is a type of interferometry systems based on a broadband light source and a bulk Michelson interferometer, which provides label-free and non-invasive visualization of biological samples. The integration between the two systems is simple because both systems use a wide-field illumination scheme and a conventional microscope. This combined imaging system gives us both functional information at a molecular level (∼20nm) and structural information at the sub-cellular level (∼1μm). For thick samples such as tissue slices, while FF-OCM is readily capable of imaging the 3D architecture, STORM suffer from aberrations and high background fluorescence that substantially degrade the resolution. In order to correct the aberrations in thick tissues, we employed an adaptive optics system in the detection path of the STORM microscope. We used our multimodal system to obtain images on brain tissue samples with structural and functional information.

Original languageEnglish
Title of host publicationMultimodal Biomedical Imaging X
EditorsFred S. Azar, Xavier Intes
PublisherSPIE
ISBN (Electronic)9781628414066
DOIs
Publication statusPublished - 2015 Jan 1
Externally publishedYes
EventMultimodal Biomedical Imaging X - San Francisco, United States
Duration: 2015 Feb 7 → …

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9316
ISSN (Print)1605-7422

Conference

ConferenceMultimodal Biomedical Imaging X
CountryUnited States
CitySan Francisco
Period15/2/7 → …

Fingerprint

Fluorescence microscopy
Fluorescence Microscopy
Microscopy
Microscopic examination
microscopy
fluorescence
Microscopes
Fluorescence
Optical Imaging
microscopes
Tissue
Aberrations
Imaging techniques
Imaging systems
aberration
Multimodal Imaging
Interferometry
Michelson interferometers
Adaptive optics
Molecular Structure

Keywords

  • Correlative imaging
  • FF-OCM
  • Fullfield Optical Coherence Microscopy
  • Multi-modal imaging
  • Stochastic optical reconstruction microscopy
  • STORM
  • Super-resolution imaging

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Kim, S., Kim, G. T., Jang, S., Shim, S-H., & Bae, S. C. (2015). Correlative super-resolution fluorescence microscopy combined with optical coherence microscopy. In F. S. Azar, & X. Intes (Eds.), Multimodal Biomedical Imaging X [93160R] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9316). SPIE. https://doi.org/10.1117/12.2079340

Correlative super-resolution fluorescence microscopy combined with optical coherence microscopy. / Kim, Sungho; Kim, Gyeong Tae; Jang, Soohyun; Shim, Sang-Hee; Bae, Sung Chul.

Multimodal Biomedical Imaging X. ed. / Fred S. Azar; Xavier Intes. SPIE, 2015. 93160R (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9316).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kim, S, Kim, GT, Jang, S, Shim, S-H & Bae, SC 2015, Correlative super-resolution fluorescence microscopy combined with optical coherence microscopy. in FS Azar & X Intes (eds), Multimodal Biomedical Imaging X., 93160R, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 9316, SPIE, Multimodal Biomedical Imaging X, San Francisco, United States, 15/2/7. https://doi.org/10.1117/12.2079340
Kim S, Kim GT, Jang S, Shim S-H, Bae SC. Correlative super-resolution fluorescence microscopy combined with optical coherence microscopy. In Azar FS, Intes X, editors, Multimodal Biomedical Imaging X. SPIE. 2015. 93160R. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.2079340
Kim, Sungho ; Kim, Gyeong Tae ; Jang, Soohyun ; Shim, Sang-Hee ; Bae, Sung Chul. / Correlative super-resolution fluorescence microscopy combined with optical coherence microscopy. Multimodal Biomedical Imaging X. editor / Fred S. Azar ; Xavier Intes. SPIE, 2015. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
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