Cotransplanted bone marrow derived mesenchymal stem cells (MSC) enhanced engraftment of hematopoietic stem cells in a MSC-dose dependent manner in NOD/SCID mice

Hyun Kim Dong, Hee Yoo Keon, Sook Yim Young, Jaewon Choi, Hyun Lee Soo, Lim Jung Hye, Woong Sung Ki, Sung Eun Yang, Il Oh Won, Yoon Sun Yang, Sang Hee Kim, Sang Yun Choi, Hoe Koo Hong

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25 Citations (Scopus)

Abstract

Transplantation of marrow-derived mesenchymal stem cells (MSCs), expanded by culture in addition to whole bone marrow, has been shown to enhance engraftment of human hematopoietic stem cells (HSCs). Our hypothesis was that there might be an optimum ratio range that could enhance engraftment. We examined the percent donor chimerism according to the ratio of HSCs to MSCs in non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice. We tested a series of ratios of co-transplanted CD34+-selected bone marrow cells, and marrow-derived MSCs into sublethally irradiated NOD/SCID mice. In all experiments, 1 × 105 bone marrow derived human CD34 + cells were administered to each mouse and human MSCs from different donors were infused concomitantly. We repeated the procedure three times and evaluated engraftment with flow cytometry four weeks after each transplantation. Serial ratios of HSCs to MSCs were 1:0, 1:1, 1:2 and 1:4, in the first experiment, 1:0, 1:1, 1:2, 1:4 and 1:8 in the second and 1:0, 1:1, 1:4, 1:8 and 1:16 in the third. Cotransplantation of HSCs and MSCs enhanced engraftment as the dose of MSCs increased. Our results suggest that the optimal ratio of HSCs and MSCs for cotransplantation might be in the range of 1:8-1:16; whereas, an excessive dose of MSCs might decrease engraftment efficiency.

Original languageEnglish
Pages (from-to)1000-1004
Number of pages5
JournalJournal of Korean medical science
Volume21
Issue number6
DOIs
Publication statusPublished - 2006

Keywords

  • Engraftment
  • Hematopoietic stem cells
  • Mesenchymal stem cells
  • Mice, SCID
  • Transplantation

ASJC Scopus subject areas

  • Medicine(all)

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    Dong, H. K., Keon, H. Y., Young, S. Y., Choi, J., Soo, H. L., Hye, L. J., Ki, W. S., Yang, S. E., Won, I. O., Yang, Y. S., Kim, S. H., Choi, S. Y., & Hong, H. K. (2006). Cotransplanted bone marrow derived mesenchymal stem cells (MSC) enhanced engraftment of hematopoietic stem cells in a MSC-dose dependent manner in NOD/SCID mice. Journal of Korean medical science, 21(6), 1000-1004. https://doi.org/10.3346/jkms.2006.21.6.1000