Coumarin-derived cu2+-selective fluorescence sensor: Synthesis, mechanisms, and applications in living cells

Hyo Sung Jung, Pil Seung Kwon, Jeong Won Lee, Jae I I Kim, Chang Seop Hong, Jong Wan Kim, Shihai Yan, Jin Yong Lee, Jung Hwa Lee, Taiha Joo, Jong Seung Kim

Research output: Contribution to journalArticlepeer-review

868 Citations (Scopus)

Abstract

A novel coumarin-based fluorogenic probe bearing the 2-picoIyI unit (1) was developed as a fluorescent chemosensor with high selectivity and suitable affinity in biological systems toward Cu2+ over other cations tested. The fluorescence on-off mechanism was studied by femtosecond time-resolved fluorescence (TRF) upconversion technique and ab initio calculations. The receptor can be applied to the monitoring of Cu2+ ion in aqueous solution with a pH span 4-10. To confirm the suitability of 1 for biological applications, we also employed it for the fluorescence detection of the changes of intracellular Cu2+ in cultured cells. The results indicate that 1 should be useful for the fluorescence microscopic imaging and the study on the biological functions of Cu2+.

Original languageEnglish
Pages (from-to)2008-2012
Number of pages5
JournalJournal of the American Chemical Society
Volume131
Issue number5
DOIs
Publication statusPublished - 2009 Feb 11

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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