Coumestrol induces mitochondrial biogenesis by activating sirt1 in cultured skeletal muscle cells

Dae Bang Seo, Hyun Woo Jeong, Sang Jun Lee, Sung-Joon Lee

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The mitochondrion is a central organelle in cellular energy homeostasis; thus, reduced mitochondrial activity has been associated with aging and metabolic disorders. This paper provides biological evidence that coumestrol, which is a natural isoflavone, activates mitochondrial biogenesis. In cultured myocytes, coumestrol activated the silent information regulator two ortholog 1 (Sirt1) through the elevation of the intracellular NAD+/NADH ratio. Coumestrol also increased the mitochondrial contents and induced the expression of key proteins in the mitochondrial electron transfer chain in cultured myocytes. A Sirt1 inhibitor and Sirt1-targeting siRNAs abolished the effect of coumestrol on mitochondrial biogenesis. Similar to an increase in mitochondrial content, coumestrol improved myocyte function with increased ATP concentration. Taken together, the data suggest that coumestrol is a novel inducer of mitochondrial biogenesis through the activation of Sirt1.

Original languageEnglish
Pages (from-to)4298-4305
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume62
Issue number19
DOIs
Publication statusPublished - 2014 May 14

Fingerprint

Coumestrol
coumestrol
Organelle Biogenesis
myocytes
Muscle Cells
Muscle
skeletal muscle
Skeletal Muscle
Cells
NAD
Mitochondria
Isoflavones
Mitochondrial Proteins
electron transport chain
metabolic diseases
isoflavones
Organelles
organelles
biogenesis
homeostasis

Keywords

  • coumestrol
  • mitochondrial biogenesis
  • myocyte
  • Sirt1

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

Coumestrol induces mitochondrial biogenesis by activating sirt1 in cultured skeletal muscle cells. / Seo, Dae Bang; Jeong, Hyun Woo; Lee, Sang Jun; Lee, Sung-Joon.

In: Journal of Agricultural and Food Chemistry, Vol. 62, No. 19, 14.05.2014, p. 4298-4305.

Research output: Contribution to journalArticle

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