Coumestrol suppresses proliferation of ES2 human epithelial ovarian cancer cells

Whasun Lim, Wooyoung Jeong, Gwonhwa Song

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Coumestrol, which is predominantly found in soybean products as a phytoestrogen, has cancer preventive activities in estrogen-responsive carcinomas. However, effects and molecular targets of coumestrol have not been reported for epithelial ovarian cancer (EOC). In the present study, we demonstrated that coumestrol inhibited viability and invasion and induced apoptosis of ES2 (clear cell-/serous carcinoma origin) cells. In addition, immunoreactive PCNA and ERBB2, markers of proliferation of ovarian carcinoma, were attenuated in their expression in coumestrol-induced death of ES2 cells. Phosphorylation of AKT, p70S6K, ERK1/2, JNK1/2, and p90RSK was inactivated by coumestrol treatment in a dose- and time-dependent manner as determined in western blot analyses. Moreover, PI3K inhibitors enhanced effects of coumestrol to decrease phosphorylation of AKT, p70S6K, S6, and ERK1/2. Furthermore, coumestrol has strong cancer preventive effects as compared to other conventional chemotherapeutics on proliferation of ES2 cells. In conclusion, coumestrol exerts chemotherapeutic effects via PI3K and ERK1/2 MAPK pathways and is a potentially novel treatment regimen with enhanced chemoprevention activities against progression of EOC.

Original languageEnglish
Pages (from-to)149-160
Number of pages12
JournalJournal of Endocrinology
Volume228
Issue number3
DOIs
Publication statusPublished - 2016 Mar 1

Fingerprint

Coumestrol
70-kDa Ribosomal Protein S6 Kinases
Carcinoma
Phosphatidylinositol 3-Kinases
Phosphorylation
S 6
Phytoestrogens
Ovarian epithelial cancer
MAP Kinase Signaling System
Chemoprevention
Proliferating Cell Nuclear Antigen
Soybeans
Neoplasms
Estrogens
Cell Death
Western Blotting
Cell Proliferation
Apoptosis

Keywords

  • clear cell carcinoma
  • coumestrol
  • ovary
  • reproduction
  • reproductive tract

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Coumestrol suppresses proliferation of ES2 human epithelial ovarian cancer cells. / Lim, Whasun; Jeong, Wooyoung; Song, Gwonhwa.

In: Journal of Endocrinology, Vol. 228, No. 3, 01.03.2016, p. 149-160.

Research output: Contribution to journalArticle

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