Abstract
Cationic lipid emulsion systems consisting of 1,2-dioleoyl-sn-glycero-3- trimethyl-ammonium-propane (DOTAP) and plasmid DNA with various counterions in the lipid headgroups were prepared. The transfection activity of the cationic lipid emulsion systems was then investigated in vitro and in vivo. The complex formation of plasmid DNA and lipid emulsion was affected by the counterions through charged headgroup repulsion and also by the salt concentration in the media. As such, the transfection activity of the DOTAP emulsion system can be controlled by changing the counterions.
| Original language | English |
|---|---|
| Pages (from-to) | 279-283 |
| Number of pages | 5 |
| Journal | Biotechnology and Bioprocess Engineering |
| Volume | 6 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2001 |
Keywords
- COS-1 cell
- Cationic lipid emulsion
- Counterion
- DOTAP
- Gene transfer
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
- Biomedical Engineering
Fingerprint Dive into the research topics of 'Counterion effects on transfection activity of cationic lipid emulsion'. Together they form a unique fingerprint.
Cite this
Kim, Y. J., Kim, T. W., Chung, H., Kwon, I. C., & Jeong, S. Y. (2001). Counterion effects on transfection activity of cationic lipid emulsion. Biotechnology and Bioprocess Engineering, 6(4), 279-283. https://doi.org/10.1007/BF02931990