Abstract
Cationic lipid emulsion systems consisting of 1,2-dioleoyl-sn-glycero-3- trimethyl-ammonium-propane (DOTAP) and plasmid DNA with various counterions in the lipid headgroups were prepared. The transfection activity of the cationic lipid emulsion systems was then investigated in vitro and in vivo. The complex formation of plasmid DNA and lipid emulsion was affected by the counterions through charged headgroup repulsion and also by the salt concentration in the media. As such, the transfection activity of the DOTAP emulsion system can be controlled by changing the counterions.
| Original language | English |
|---|---|
| Pages (from-to) | 279-283 |
| Number of pages | 5 |
| Journal | Biotechnology and Bioprocess Engineering |
| Volume | 6 |
| Issue number | 4 |
| Publication status | Published - 2001 Dec 1 |
| Externally published | Yes |
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Keywords
- Cationic lipid emulsion
- COS-1 cell
- Counterion
- DOTAP
- Gene transfer
ASJC Scopus subject areas
- Biotechnology
- Biomedical Engineering
- Applied Microbiology and Biotechnology
Cite this
Counterion effects on transfection activity of cationic lipid emulsion. / Kim, Young Jin; Kim, Tae Woo; Chung, Hesson; Kwon, Ick Chan; Jeong, Seo Young.
In: Biotechnology and Bioprocess Engineering, Vol. 6, No. 4, 01.12.2001, p. 279-283.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Counterion effects on transfection activity of cationic lipid emulsion
AU - Kim, Young Jin
AU - Kim, Tae Woo
AU - Chung, Hesson
AU - Kwon, Ick Chan
AU - Jeong, Seo Young
PY - 2001/12/1
Y1 - 2001/12/1
N2 - Cationic lipid emulsion systems consisting of 1,2-dioleoyl-sn-glycero-3- trimethyl-ammonium-propane (DOTAP) and plasmid DNA with various counterions in the lipid headgroups were prepared. The transfection activity of the cationic lipid emulsion systems was then investigated in vitro and in vivo. The complex formation of plasmid DNA and lipid emulsion was affected by the counterions through charged headgroup repulsion and also by the salt concentration in the media. As such, the transfection activity of the DOTAP emulsion system can be controlled by changing the counterions.
AB - Cationic lipid emulsion systems consisting of 1,2-dioleoyl-sn-glycero-3- trimethyl-ammonium-propane (DOTAP) and plasmid DNA with various counterions in the lipid headgroups were prepared. The transfection activity of the cationic lipid emulsion systems was then investigated in vitro and in vivo. The complex formation of plasmid DNA and lipid emulsion was affected by the counterions through charged headgroup repulsion and also by the salt concentration in the media. As such, the transfection activity of the DOTAP emulsion system can be controlled by changing the counterions.
KW - Cationic lipid emulsion
KW - COS-1 cell
KW - Counterion
KW - DOTAP
KW - Gene transfer
UR - http://www.scopus.com/inward/record.url?scp=0012874322&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0012874322&partnerID=8YFLogxK
M3 - Article
VL - 6
SP - 279
EP - 283
JO - Biotechnology and Bioprocess Engineering
JF - Biotechnology and Bioprocess Engineering
SN - 1226-8372
IS - 4
ER -