Counterion effects on transfection activity of cationic lipid emulsion

Young Jin Kim, Tae Woo Kim, Hesson Chung, Ik Chan Kwon, Seo Young Jeong

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Cationic lipid emulsion systems consisting of 1,2-dioleoyl-sn-glycero-3- trimethyl-ammonium-propane (DOTAP) and plasmid DNA with various counterions in the lipid headgroups were prepared. The transfection activity of the cationic lipid emulsion systems was then investigated in vitro and in vivo. The complex formation of plasmid DNA and lipid emulsion was affected by the counterions through charged headgroup repulsion and also by the salt concentration in the media. As such, the transfection activity of the DOTAP emulsion system can be controlled by changing the counterions.

Original languageEnglish
Pages (from-to)279-283
Number of pages5
JournalBiotechnology and Bioprocess Engineering
Volume6
Issue number4
DOIs
Publication statusPublished - 2001 Jan 1

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Keywords

  • COS-1 cell
  • Cationic lipid emulsion
  • Counterion
  • DOTAP
  • Gene transfer

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering

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