Counterion effects on transfection activity of cationic lipid emulsion

Young Jin Kim; Tae Woo Kim; Hesson Chung; Ik Chan Kwon; Seo Young Jeong

doi:10.1007/BF02931990

Counterion effects on transfection activity of cationic lipid emulsion

Young Jin Kim, Tae Woo Kim, Hesson Chung, Ik Chan Kwon, Seo Young Jeong

Research output: Contribution to journal › Article

7 Citations (Scopus)

Abstract

Cationic lipid emulsion systems consisting of 1,2-dioleoyl-sn-glycero-3- trimethyl-ammonium-propane (DOTAP) and plasmid DNA with various counterions in the lipid headgroups were prepared. The transfection activity of the cationic lipid emulsion systems was then investigated in vitro and in vivo. The complex formation of plasmid DNA and lipid emulsion was affected by the counterions through charged headgroup repulsion and also by the salt concentration in the media. As such, the transfection activity of the DOTAP emulsion system can be controlled by changing the counterions.

Original language	English
Pages (from-to)	279-283
Number of pages	5
Journal	Biotechnology and Bioprocess Engineering
Volume	6
Issue number	4
DOIs	https://doi.org/10.1007/BF02931990
Publication status	Published - 2001 Jan 1

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Keywords

COS-1 cell
Cationic lipid emulsion
Counterion
DOTAP
Gene transfer

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology
Biomedical Engineering

Cite this

Kim, Y. J., Kim, T. W., Chung, H., Kwon, I. C., & Jeong, S. Y. (2001). Counterion effects on transfection activity of cationic lipid emulsion. Biotechnology and Bioprocess Engineering, 6(4), 279-283. https://doi.org/10.1007/BF02931990

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Access to Document

10.1007/BF02931990