Counterion effects on transfection activity of cationic lipid emulsion

Young Jin Kim; Tae Woo Kim; Hesson Chung; Ik Chan Kwon; Seo Young Jeong

doi:10.1007/BF02931990

Counterion effects on transfection activity of cationic lipid emulsion

Young Jin Kim, Tae Woo Kim, Hesson Chung, Ik Chan Kwon, Seo Young Jeong

KU-KIST Graduate School of Converging Science and Technology

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Cationic lipid emulsion systems consisting of 1,2-dioleoyl-sn-glycero-3- trimethyl-ammonium-propane (DOTAP) and plasmid DNA with various counterions in the lipid headgroups were prepared. The transfection activity of the cationic lipid emulsion systems was then investigated in vitro and in vivo. The complex formation of plasmid DNA and lipid emulsion was affected by the counterions through charged headgroup repulsion and also by the salt concentration in the media. As such, the transfection activity of the DOTAP emulsion system can be controlled by changing the counterions.

Original language	English
Pages (from-to)	279-283
Number of pages	5
Journal	Biotechnology and Bioprocess Engineering
Volume	6
Issue number	4
DOIs	https://doi.org/10.1007/BF02931990
Publication status	Published - 2001

Keywords

COS-1 cell
Cationic lipid emulsion
Counterion
DOTAP
Gene transfer

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology
Biomedical Engineering

Access to Document

10.1007/BF02931990

Fingerprint Dive into the research topics of 'Counterion effects on transfection activity of cationic lipid emulsion'. Together they form a unique fingerprint.

Cite this

@article{47461a5f0ea449aa8f21ef7d1fbdd064,

title = "Counterion effects on transfection activity of cationic lipid emulsion",

abstract = "Cationic lipid emulsion systems consisting of 1,2-dioleoyl-sn-glycero-3- trimethyl-ammonium-propane (DOTAP) and plasmid DNA with various counterions in the lipid headgroups were prepared. The transfection activity of the cationic lipid emulsion systems was then investigated in vitro and in vivo. The complex formation of plasmid DNA and lipid emulsion was affected by the counterions through charged headgroup repulsion and also by the salt concentration in the media. As such, the transfection activity of the DOTAP emulsion system can be controlled by changing the counterions.",

keywords = "COS-1 cell, Cationic lipid emulsion, Counterion, DOTAP, Gene transfer",

author = "Kim, {Young Jin} and Kim, {Tae Woo} and Hesson Chung and Kwon, {Ik Chan} and Jeong, {Seo Young}",

year = "2001",

doi = "10.1007/BF02931990",

language = "English",

volume = "6",

pages = "279--283",

journal = "Biotechnology and Bioprocess Engineering",

issn = "1226-8372",

publisher = "Korean Society for Biotechnology and Bioengineering",

number = "4",

}

TY - JOUR

T1 - Counterion effects on transfection activity of cationic lipid emulsion

AU - Kim, Young Jin

AU - Kim, Tae Woo

AU - Chung, Hesson

AU - Kwon, Ik Chan

AU - Jeong, Seo Young

PY - 2001

Y1 - 2001

N2 - Cationic lipid emulsion systems consisting of 1,2-dioleoyl-sn-glycero-3- trimethyl-ammonium-propane (DOTAP) and plasmid DNA with various counterions in the lipid headgroups were prepared. The transfection activity of the cationic lipid emulsion systems was then investigated in vitro and in vivo. The complex formation of plasmid DNA and lipid emulsion was affected by the counterions through charged headgroup repulsion and also by the salt concentration in the media. As such, the transfection activity of the DOTAP emulsion system can be controlled by changing the counterions.

AB - Cationic lipid emulsion systems consisting of 1,2-dioleoyl-sn-glycero-3- trimethyl-ammonium-propane (DOTAP) and plasmid DNA with various counterions in the lipid headgroups were prepared. The transfection activity of the cationic lipid emulsion systems was then investigated in vitro and in vivo. The complex formation of plasmid DNA and lipid emulsion was affected by the counterions through charged headgroup repulsion and also by the salt concentration in the media. As such, the transfection activity of the DOTAP emulsion system can be controlled by changing the counterions.

KW - COS-1 cell

KW - Cationic lipid emulsion

KW - Counterion

KW - DOTAP

KW - Gene transfer

UR - http://www.scopus.com/inward/record.url?scp=0012874322&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0012874322&partnerID=8YFLogxK

U2 - 10.1007/BF02931990

DO - 10.1007/BF02931990

M3 - Article

AN - SCOPUS:0012874322

VL - 6

SP - 279

EP - 283

JO - Biotechnology and Bioprocess Engineering

JF - Biotechnology and Bioprocess Engineering

SN - 1226-8372

IS - 4

ER -