Counterion effects on transfection activity of cationic lipid emulsion

Young Jin Kim, Tae Woo Kim, Hesson Chung, Ick Chan Kwon, Seo Young Jeong

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Cationic lipid emulsion systems consisting of 1,2-dioleoyl-sn-glycero-3- trimethyl-ammonium-propane (DOTAP) and plasmid DNA with various counterions in the lipid headgroups were prepared. The transfection activity of the cationic lipid emulsion systems was then investigated in vitro and in vivo. The complex formation of plasmid DNA and lipid emulsion was affected by the counterions through charged headgroup repulsion and also by the salt concentration in the media. As such, the transfection activity of the DOTAP emulsion system can be controlled by changing the counterions.

Original languageEnglish
Pages (from-to)279-283
Number of pages5
JournalBiotechnology and Bioprocess Engineering
Volume6
Issue number4
Publication statusPublished - 2001 Dec 1
Externally publishedYes

Fingerprint

Emulsions
Lipids
Transfection
Propane
Ammonium Compounds
Plasmids
DNA
Salts

Keywords

  • Cationic lipid emulsion
  • COS-1 cell
  • Counterion
  • DOTAP
  • Gene transfer

ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering
  • Applied Microbiology and Biotechnology

Cite this

Counterion effects on transfection activity of cationic lipid emulsion. / Kim, Young Jin; Kim, Tae Woo; Chung, Hesson; Kwon, Ick Chan; Jeong, Seo Young.

In: Biotechnology and Bioprocess Engineering, Vol. 6, No. 4, 01.12.2001, p. 279-283.

Research output: Contribution to journalArticle

Kim, Young Jin ; Kim, Tae Woo ; Chung, Hesson ; Kwon, Ick Chan ; Jeong, Seo Young. / Counterion effects on transfection activity of cationic lipid emulsion. In: Biotechnology and Bioprocess Engineering. 2001 ; Vol. 6, No. 4. pp. 279-283.
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