Covalent linkage of IL-12 and ovalbumin confines the effects of IL-12 to ovalbumin-specific immune responses

Tae Sung Kim, Seung Yong Hwang, Gyurng Soo Yoo

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

In order to direct the form of the immune response in an antigen-specific manner, we constructed a fusion protein (OVA/IL12) that contained the T cell-dependent antigen, ovalbumin (OVA), covalently linked to murine interleukin-12 (IL-12). The OVA/IL12 protein was produced in a baculovirus expression system and was purified by anti-OVA immunoaffinity chromatography. The purified OVA/IL12 protein displayed potent IL-12 bioactivity in an IL-12 proliferation assay. BALB/c mice immunized with the OVA/IL12 protein produced increased quantities of anti-OVA IgG2a antibody compared with mice immunized with recombinant OVA alone. Lymph node cells from the immunized mice with the OVA/IL12 protein produced large amounts of IFN-γ when restimulated in vitro with OVA, while those from mice immunized with the OVA protein produced little or no IFN-γ. In contrast, immunization with a mixture of OVA and free recombinant IL-12 also induced IFN-γ production, which was not OVA-specific. These studies indicate that the OVA/IL12 fusion protein can induce OVA-specific, Th1-dominated immune responses, and that the covalent linkage of OVA and IL-12 confines the effect of IL-12 to OVA-specific cells.

Original languageEnglish
Pages (from-to)396-403
Number of pages8
JournalArchives of pharmacal research
Volume20
Issue number5
DOIs
Publication statusPublished - 1997 Oct
Externally publishedYes

Keywords

  • Allergy
  • Antigen-specific
  • Interferon-γ
  • Interleukin-12
  • Ovabumin
  • Th cell

ASJC Scopus subject areas

  • Molecular Medicine
  • Drug Discovery
  • Organic Chemistry

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