TY - JOUR
T1 - Covalently grafted 2-methacryloyloxyethyl phosphorylcholine networks inhibit fibrous capsule formation around silicone breast implants in a porcine model
AU - Park, Ji Ung
AU - Lee, Yan
AU - Ham, Jiyeon
AU - Kim, Youngmin
AU - An, Taeyang
AU - Kang, Sunah
AU - Ha, Cheolmin
AU - Wufue, Maierdanjiang
AU - Kim, Yumin
AU - Jeon, Byoungjun
AU - Kim, Seulah
AU - Kim, Jungah
AU - Choi, Tae Hyun
AU - Seo, Ji Hun
AU - Kim, Dae Woo
N1 - Funding Information:
This work was supported by the National Research Foundation of Korea Grant (NRF-2017M3A9E9072938, NRF-2017M3A9E9072939, and NRF-2018M3C1B7020722), the Korea Health Technology R&D Project (HR18C0006), the Korea Health Industry Development Institute (KHIDI), the Ministry of Health & Welfare, Korea, Baeksan Co. Ltd., and BS research Co. Ltd. Korea.
Publisher Copyright:
© 2020 American Chemical Society.
PY - 2020/7/8
Y1 - 2020/7/8
N2 - The surface of human silicone breast implants is covalently grafted at a high density with a 2-methacryloyloxyethyl phosphorylcholine (MPC)-based polymer. Addition of crosslinkers is essential for enhancing the density and mechanical durability of the MPC graft. The MPC graft strongly inhibits not only adsorption but also the conformational deformation of fibrinogen, resulting in the exposure of a buried amino acid sequence, γ377-395, which is recognized by inflammatory cells. Furthermore, the numbers of adhered macrophages and the amounts of released cytokines (MIP-1α, MIP-1β, IL-8, TNFα, IL- 1α, IL-1β, and IL-10) are dramatically decreased when the MPC network is introduced at a high density on the silicone surface (cross-linked PMPC-silicone). We insert the MPC-grafted human silicone breast implants into Yorkshire pigs to analyze the in vivo effect of the MPC graft on the capsular formation around the implants. After 6 month implantation, marked reductions of inflammatory cell recruitment, inflammatory-related proteins (TGF-β and myeloperoxidase), a myoblast marker (α-smooth muscle actin), vascularity-related factors (blood vessels and VEGF), and, most importantly, capsular thickness are observed on the crosslinked PMPC-silicone. We propose a mechanism of the MPC grafting effect on fibrous capsular formation around silicone implants on the basis of the in vitro and in vivo results.
AB - The surface of human silicone breast implants is covalently grafted at a high density with a 2-methacryloyloxyethyl phosphorylcholine (MPC)-based polymer. Addition of crosslinkers is essential for enhancing the density and mechanical durability of the MPC graft. The MPC graft strongly inhibits not only adsorption but also the conformational deformation of fibrinogen, resulting in the exposure of a buried amino acid sequence, γ377-395, which is recognized by inflammatory cells. Furthermore, the numbers of adhered macrophages and the amounts of released cytokines (MIP-1α, MIP-1β, IL-8, TNFα, IL- 1α, IL-1β, and IL-10) are dramatically decreased when the MPC network is introduced at a high density on the silicone surface (cross-linked PMPC-silicone). We insert the MPC-grafted human silicone breast implants into Yorkshire pigs to analyze the in vivo effect of the MPC graft on the capsular formation around the implants. After 6 month implantation, marked reductions of inflammatory cell recruitment, inflammatory-related proteins (TGF-β and myeloperoxidase), a myoblast marker (α-smooth muscle actin), vascularity-related factors (blood vessels and VEGF), and, most importantly, capsular thickness are observed on the crosslinked PMPC-silicone. We propose a mechanism of the MPC grafting effect on fibrous capsular formation around silicone implants on the basis of the in vitro and in vivo results.
KW - Capsular formation
KW - Inhibition mechanism
KW - Phosphorylcholine-based polymer
KW - Porcine model
KW - Silicone breast implants
UR - http://www.scopus.com/inward/record.url?scp=85088207287&partnerID=8YFLogxK
U2 - 10.1021/acsami.0c07629
DO - 10.1021/acsami.0c07629
M3 - Article
C2 - 32574031
AN - SCOPUS:85088207287
VL - 12
SP - 30198
EP - 30212
JO - ACS applied materials & interfaces
JF - ACS applied materials & interfaces
SN - 1944-8244
IS - 27
ER -