Covalently grafted 2-methacryloyloxyethyl phosphorylcholine networks inhibit fibrous capsule formation around silicone breast implants in a porcine model

Ji Ung Park, Yan Lee, Jiyeon Ham, Youngmin Kim, Taeyang An, Sunah Kang, Cheolmin Ha, Maierdanjiang Wufue, Yumin Kim, Byoungjun Jeon, Seulah Kim, Jungah Kim, Tae Hyun Choi, Ji Hun Seo, Dae Woo Kim

Research output: Contribution to journalArticlepeer-review

Abstract

The surface of human silicone breast implants is covalently grafted at a high density with a 2-methacryloyloxyethyl phosphorylcholine (MPC)-based polymer. Addition of crosslinkers is essential for enhancing the density and mechanical durability of the MPC graft. The MPC graft strongly inhibits not only adsorption but also the conformational deformation of fibrinogen, resulting in the exposure of a buried amino acid sequence, γ377-395, which is recognized by inflammatory cells. Furthermore, the numbers of adhered macrophages and the amounts of released cytokines (MIP-1α, MIP-1β, IL-8, TNFα, IL- 1α, IL-1β, and IL-10) are dramatically decreased when the MPC network is introduced at a high density on the silicone surface (cross-linked PMPC-silicone). We insert the MPC-grafted human silicone breast implants into Yorkshire pigs to analyze the in vivo effect of the MPC graft on the capsular formation around the implants. After 6 month implantation, marked reductions of inflammatory cell recruitment, inflammatory-related proteins (TGF-β and myeloperoxidase), a myoblast marker (α-smooth muscle actin), vascularity-related factors (blood vessels and VEGF), and, most importantly, capsular thickness are observed on the crosslinked PMPC-silicone. We propose a mechanism of the MPC grafting effect on fibrous capsular formation around silicone implants on the basis of the in vitro and in vivo results.

Original languageEnglish
Pages (from-to)30198-30212
Number of pages15
JournalACS Applied Materials and Interfaces
Volume12
Issue number27
DOIs
Publication statusPublished - 2020 Jul 8

Keywords

  • Capsular formation
  • Inhibition mechanism
  • Phosphorylcholine-based polymer
  • Porcine model
  • Silicone breast implants

ASJC Scopus subject areas

  • Materials Science(all)

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