CRE-decoy oligonucleotide-inhibition of gene expression and tumor growth

Yoon S. Cho-Chung, Yun Gyu Park, Maria Nesterova, Youl Nam Lee, Yee Sook Cho

Research output: Contribution to journalReview article

34 Citations (Scopus)

Abstract

Nucleic acid molecules with high affinities for a target transcription factor can be introduced into cells as decoy cis-elements to bind these factors and alter gene expression. This review discusses a synthetic single-stranded palindromic oligonucleotide, which self-hybridizes to form a duplex/hairpin and competes with cAMP response element (CRE) enhancers for binding transcription factors. This oligonucleotide inhibits CRE- and Ap-1-directed gene transcription and promotes growth inhibition in vitro and in vivo in a broad spectrum of cancer cells, without adversely affecting normal cell growth. Evidence presented here suggests that the CRE-decoy oligonucleotide can provide a powerful new means of combating cancers, viral diseases, and other pathological conditions by regulating the expression of cAMP-responsive genes.

Original languageEnglish
Pages (from-to)29-34
Number of pages6
JournalMolecular and Cellular Biochemistry
Volume212
Issue number1-2
DOIs
Publication statusPublished - 2000 Jan 1
Externally publishedYes

    Fingerprint

Keywords

  • Ap-1
  • cAMP
  • CRE
  • Gene expression
  • p53
  • Transcription factor-decoy oligonucleotides
  • Tumor growth

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this