CRE-decoy oligonucleotide-inhibition of gene expression and tumor growth

Yoon S. Cho-Chung, Yun Gyu Park, Maria Nesterova, Youl Nam Lee, Yee Sook Cho

Research output: Contribution to journalReview article

34 Citations (Scopus)

Abstract

Nucleic acid molecules with high affinities for a target transcription factor can be introduced into cells as decoy cis-elements to bind these factors and alter gene expression. This review discusses a synthetic single-stranded palindromic oligonucleotide, which self-hybridizes to form a duplex/hairpin and competes with cAMP response element (CRE) enhancers for binding transcription factors. This oligonucleotide inhibits CRE- and Ap-1-directed gene transcription and promotes growth inhibition in vitro and in vivo in a broad spectrum of cancer cells, without adversely affecting normal cell growth. Evidence presented here suggests that the CRE-decoy oligonucleotide can provide a powerful new means of combating cancers, viral diseases, and other pathological conditions by regulating the expression of cAMP-responsive genes.

Original languageEnglish
Pages (from-to)29-34
Number of pages6
JournalMolecular and Cellular Biochemistry
Volume212
Issue number1-2
DOIs
Publication statusPublished - 2000 Jan 1
Externally publishedYes

Fingerprint

Response Elements
Gene expression
Oligonucleotides
Tumors
Gene Expression
Transcription Factors
Growth
Genes
Neoplasms
Cell growth
Virus Diseases
Transcription
Nucleic Acids
Cells
Molecules

Keywords

  • Ap-1
  • cAMP
  • CRE
  • Gene expression
  • p53
  • Transcription factor-decoy oligonucleotides
  • Tumor growth

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

CRE-decoy oligonucleotide-inhibition of gene expression and tumor growth. / Cho-Chung, Yoon S.; Park, Yun Gyu; Nesterova, Maria; Lee, Youl Nam; Cho, Yee Sook.

In: Molecular and Cellular Biochemistry, Vol. 212, No. 1-2, 01.01.2000, p. 29-34.

Research output: Contribution to journalReview article

Cho-Chung, Yoon S. ; Park, Yun Gyu ; Nesterova, Maria ; Lee, Youl Nam ; Cho, Yee Sook. / CRE-decoy oligonucleotide-inhibition of gene expression and tumor growth. In: Molecular and Cellular Biochemistry. 2000 ; Vol. 212, No. 1-2. pp. 29-34.
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