Creating a New Pathway in Corynebacterium glutamicum for the Production of Taurine as a Food Additive

Young Chul Joo, Young Jin Ko, Seung Kyou You, Sang Kyu Shin, Jeong Eun Hyeon, Almisned Shuaa Musaad, Sung Ok Han

Research output: Contribution to journalArticle

Abstract

Taurine is a biologically and physiologically valuable food additive. However, commercial taurine production mainly relies on environmentally harmful chemical synthesis. Herein, for the first time in bacteria, we attempted to produce taurine in metabolically engineered Corynebacterium glutamicum. The taurine-producing strain was developed by introducing cs, cdo1, and csad genes. Interestingly, while the control strain could not produce taurine, the engineered strains successfully produced taurine via the newly introduced metabolic pathway. Furthermore, we investigated the effect of a deletion strain of the transcriptional repressor McbR gene on taurine production. As a result, sulfur accumulation and l-cysteine biosynthesis were reinforced by the McbR deletion strain, which further increased the taurine production by 2.3-fold. Taurine production of the final engineered strain Tau11 was higher than in other previously reported strains. This study demonstrated a potential approach for eco-friendly biosynthesis as an alternative to the chemical synthesis of a food additive.

Original languageEnglish
Pages (from-to)13454-13463
Number of pages10
JournalJournal of Agricultural and Food Chemistry
Volume66
Issue number51
DOIs
Publication statusPublished - 2018 Dec 26

Fingerprint

Food additives
Corynebacterium glutamicum
Food Additives
Taurine
taurine
food additives
Biosynthesis
Genes
biosynthesis
Strain control
synthesis
Metabolic Networks and Pathways
Sulfur
Cysteine
cysteine
biochemical pathways
Bacteria
sulfur
genes

Keywords

  • Corynebacterium glutamicum
  • food additive
  • metabolic engineering
  • new pathway
  • taurine production

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Creating a New Pathway in Corynebacterium glutamicum for the Production of Taurine as a Food Additive. / Joo, Young Chul; Ko, Young Jin; You, Seung Kyou; Shin, Sang Kyu; Hyeon, Jeong Eun; Musaad, Almisned Shuaa; Han, Sung Ok.

In: Journal of Agricultural and Food Chemistry, Vol. 66, No. 51, 26.12.2018, p. 13454-13463.

Research output: Contribution to journalArticle

Joo, Young Chul ; Ko, Young Jin ; You, Seung Kyou ; Shin, Sang Kyu ; Hyeon, Jeong Eun ; Musaad, Almisned Shuaa ; Han, Sung Ok. / Creating a New Pathway in Corynebacterium glutamicum for the Production of Taurine as a Food Additive. In: Journal of Agricultural and Food Chemistry. 2018 ; Vol. 66, No. 51. pp. 13454-13463.
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