Abstract
Taurine is a biologically and physiologically valuable food additive. However, commercial taurine production mainly relies on environmentally harmful chemical synthesis. Herein, for the first time in bacteria, we attempted to produce taurine in metabolically engineered Corynebacterium glutamicum. The taurine-producing strain was developed by introducing cs, cdo1, and csad genes. Interestingly, while the control strain could not produce taurine, the engineered strains successfully produced taurine via the newly introduced metabolic pathway. Furthermore, we investigated the effect of a deletion strain of the transcriptional repressor McbR gene on taurine production. As a result, sulfur accumulation and l-cysteine biosynthesis were reinforced by the McbR deletion strain, which further increased the taurine production by 2.3-fold. Taurine production of the final engineered strain Tau11 was higher than in other previously reported strains. This study demonstrated a potential approach for eco-friendly biosynthesis as an alternative to the chemical synthesis of a food additive.
| Original language | English |
|---|---|
| Pages (from-to) | 13454-13463 |
| Number of pages | 10 |
| Journal | Journal of Agricultural and Food Chemistry |
| Volume | 66 |
| Issue number | 51 |
| DOIs | |
| Publication status | Published - 2018 Dec 26 |
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Keywords
- Corynebacterium glutamicum
- food additive
- metabolic engineering
- new pathway
- taurine production
ASJC Scopus subject areas
- Chemistry(all)
- Agricultural and Biological Sciences(all)
Cite this
Creating a New Pathway in Corynebacterium glutamicum for the Production of Taurine as a Food Additive. / Joo, Young Chul; Ko, Young Jin; You, Seung Kyou; Shin, Sang Kyu; Hyeon, Jeong Eun; Musaad, Almisned Shuaa; Han, Sung Ok.
In: Journal of Agricultural and Food Chemistry, Vol. 66, No. 51, 26.12.2018, p. 13454-13463.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Creating a New Pathway in Corynebacterium glutamicum for the Production of Taurine as a Food Additive
AU - Joo, Young Chul
AU - Ko, Young Jin
AU - You, Seung Kyou
AU - Shin, Sang Kyu
AU - Hyeon, Jeong Eun
AU - Musaad, Almisned Shuaa
AU - Han, Sung Ok
PY - 2018/12/26
Y1 - 2018/12/26
N2 - Taurine is a biologically and physiologically valuable food additive. However, commercial taurine production mainly relies on environmentally harmful chemical synthesis. Herein, for the first time in bacteria, we attempted to produce taurine in metabolically engineered Corynebacterium glutamicum. The taurine-producing strain was developed by introducing cs, cdo1, and csad genes. Interestingly, while the control strain could not produce taurine, the engineered strains successfully produced taurine via the newly introduced metabolic pathway. Furthermore, we investigated the effect of a deletion strain of the transcriptional repressor McbR gene on taurine production. As a result, sulfur accumulation and l-cysteine biosynthesis were reinforced by the McbR deletion strain, which further increased the taurine production by 2.3-fold. Taurine production of the final engineered strain Tau11 was higher than in other previously reported strains. This study demonstrated a potential approach for eco-friendly biosynthesis as an alternative to the chemical synthesis of a food additive.
AB - Taurine is a biologically and physiologically valuable food additive. However, commercial taurine production mainly relies on environmentally harmful chemical synthesis. Herein, for the first time in bacteria, we attempted to produce taurine in metabolically engineered Corynebacterium glutamicum. The taurine-producing strain was developed by introducing cs, cdo1, and csad genes. Interestingly, while the control strain could not produce taurine, the engineered strains successfully produced taurine via the newly introduced metabolic pathway. Furthermore, we investigated the effect of a deletion strain of the transcriptional repressor McbR gene on taurine production. As a result, sulfur accumulation and l-cysteine biosynthesis were reinforced by the McbR deletion strain, which further increased the taurine production by 2.3-fold. Taurine production of the final engineered strain Tau11 was higher than in other previously reported strains. This study demonstrated a potential approach for eco-friendly biosynthesis as an alternative to the chemical synthesis of a food additive.
KW - Corynebacterium glutamicum
KW - food additive
KW - metabolic engineering
KW - new pathway
KW - taurine production
UR - http://www.scopus.com/inward/record.url?scp=85059542527&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85059542527&partnerID=8YFLogxK
U2 - 10.1021/acs.jafc.8b05093
DO - 10.1021/acs.jafc.8b05093
M3 - Article
C2 - 30516051
AN - SCOPUS:85059542527
VL - 66
SP - 13454
EP - 13463
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
SN - 0021-8561
IS - 51
ER -