Critical role of peroxisome proliferator activated receptor-δ on body fat reduction in C57BL/6J and human apolipoprotein E2 transgenic mice fed delipidated soybean

Ji Hae Lee, Hee Jin Jun, Yaoyao Jia, Wook Kim, Sung Gil Choi, Sung-Joon Lee

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The consumption of soy protein and fiber reduces body fat accumulation; however, the mechanism of this effect has not been clearly understood. We investigated the antiobesogenic effect of soy protein and fiber in two different mouse models. Normolipidemic nonobese C57BL/6J and hyperlipidemic obese human apolipoprotein E2 transgenic mice were fed either delipidated soybean (DLSB) containing soy protein and fiber or a control diet. The DLSB-fed mice showed a significant reduction in body weight gain and adiposity compared with controls, in both C57BL/6J and apoE2 mice. All metabolic parameters were significantly improved in the DLSB group compared with controls: total cholesterol, low-density lipoprotein cholesterol, insulin, and leptin levels were significantly reduced. Adiponectin concentrations were significantly elevated, and glucose tolerance was improved. In both types of DLSB-fed mice, the specific induction of PPAR-δ protein expression was evident in muscle and adipose tissues. The expression of PPAR-δ target genes in the DLSB-fed mice was also significantly altered. Acetyl-CoA carboxylase-1 and fatty acid synthase levels in adipose tissue were downregulated, and uncoupling protein-2 in muscle was upregulated. Intestinal expression of fatty acid transport protein-4, cluster of differentiation-36, and acyl-CoA synthetase were significantly downregulated. We propose that marked activation of PPAR-δ is the primary mechanism mediating the antiobesogenic effect of soybean and that PPAR-δ has multiple actions: induction of thermogenesis in muscle, reduction of fatty acid synthesis in adipose tissue, and reduction of fatty acid uptake in intestinal tissue.

Original languageEnglish
Pages (from-to)11872-11881
Number of pages10
JournalJournal of Agricultural and Food Chemistry
Volume59
Issue number21
DOIs
Publication statusPublished - 2011 Nov 9

Fingerprint

apolipoprotein E2
Apolipoprotein E2
Peroxisome Proliferator-Activated Receptors
Soybeans
Transgenic Mice
body fat
Soybean Proteins
Adipose Tissue
Fats
genetically modified organisms
Tissue
soybeans
Muscle
mice
soy protein
adipose tissue
Fibers
Fatty Acid Transport Proteins
dietary fiber
Fatty Acids

Keywords

  • adiponectin
  • delipidated soybean
  • obesity
  • peroxisome proliferator-activated receptor-δ
  • uncoupling protein

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

Critical role of peroxisome proliferator activated receptor-δ on body fat reduction in C57BL/6J and human apolipoprotein E2 transgenic mice fed delipidated soybean. / Lee, Ji Hae; Jun, Hee Jin; Jia, Yaoyao; Kim, Wook; Choi, Sung Gil; Lee, Sung-Joon.

In: Journal of Agricultural and Food Chemistry, Vol. 59, No. 21, 09.11.2011, p. 11872-11881.

Research output: Contribution to journalArticle

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