Crosslinked chitosan coating on magnetic mesoporous silica with pre-adsorbed carbonic anhydrase for carbon dioxide conversion

Kie Moon Woo, Inseon Lee, Sung Gil Hong, Sunhyung An, Jinwoo Lee, Euichaul Oh, Jungbae Kim

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Carbonic anhydrase (CA), an enzyme converting CO2 to bicarbonate, was adsorbed into magnetically-separable spherical mesocellular siliceous foam (Mag-S-MCF). Then, chitosan was adsorbed onto the surface of Mag-S-MCF with pre-adsorbed enzymes, and further crosslinked via the glutaraldehyde treatment. The resulting composite materials, the crosslinked chitosan coating on Mag-S-MCF with pre-adsorbed CA (ADS-CA/CS-GA), could effectively prevent leaching of enzymes, showing no decrease of enzyme activity under shaking (200rpm) for 85days. ADS-CA/CS-GA also showed no activity decrease under recycled uses via facile magnetic separation, and could be successfully used for the biocatalytic CO2 conversion to bicarbonate, which was further utilized to generate calcium carbonate in the second batch reactor.

Original languageEnglish
Pages (from-to)232-239
Number of pages8
JournalChemical Engineering Journal
Volume276
DOIs
Publication statusPublished - 2015 Sep 5

Keywords

  • Calcium carbonate
  • Carbon conversion and utilization
  • Carbonic anhydrase
  • Crosslinked chitosan coating
  • Enzyme immobilization and stabilization
  • Magnetic mesoporous silica

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering

Fingerprint

Dive into the research topics of 'Crosslinked chitosan coating on magnetic mesoporous silica with pre-adsorbed carbonic anhydrase for carbon dioxide conversion'. Together they form a unique fingerprint.

Cite this