Crosslinked chitosan coating on magnetic mesoporous silica with pre-adsorbed carbonic anhydrase for carbon dioxide conversion

Kie Moon Woo; Inseon Lee; Sung Gil Hong; Sunhyung An; Jinwoo Lee; Euichaul Oh; Jungbae Kim

doi:10.1016/j.cej.2015.04.057

Crosslinked chitosan coating on magnetic mesoporous silica with pre-adsorbed carbonic anhydrase for carbon dioxide conversion

Kie Moon Woo, Inseon Lee, Sung Gil Hong, Sunhyung An, Jinwoo Lee, Euichaul Oh, Jungbae Kim

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article

12 Citations (Scopus)

Abstract

Carbonic anhydrase (CA), an enzyme converting CO<inf>2</inf> to bicarbonate, was adsorbed into magnetically-separable spherical mesocellular siliceous foam (Mag-S-MCF). Then, chitosan was adsorbed onto the surface of Mag-S-MCF with pre-adsorbed enzymes, and further crosslinked via the glutaraldehyde treatment. The resulting composite materials, the crosslinked chitosan coating on Mag-S-MCF with pre-adsorbed CA (ADS-CA/CS-GA), could effectively prevent leaching of enzymes, showing no decrease of enzyme activity under shaking (200rpm) for 85days. ADS-CA/CS-GA also showed no activity decrease under recycled uses via facile magnetic separation, and could be successfully used for the biocatalytic CO<inf>2</inf> conversion to bicarbonate, which was further utilized to generate calcium carbonate in the second batch reactor.

Original language	English
Pages (from-to)	232-239
Number of pages	8
Journal	Chemical Engineering Journal
Volume	276
DOIs	https://doi.org/10.1016/j.cej.2015.04.057
Publication status	Published - 2015 Sep 5

Keywords

Calcium carbonate
Carbon conversion and utilization
Carbonic anhydrase
Crosslinked chitosan coating
Enzyme immobilization and stabilization
Magnetic mesoporous silica

ASJC Scopus subject areas

Chemical Engineering(all)
Chemistry(all)
Industrial and Manufacturing Engineering
Environmental Chemistry

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@article{4879a014e32c4faeac4cdfb762579062,

title = "Crosslinked chitosan coating on magnetic mesoporous silica with pre-adsorbed carbonic anhydrase for carbon dioxide conversion",

abstract = "Carbonic anhydrase (CA), an enzyme converting CO2 to bicarbonate, was adsorbed into magnetically-separable spherical mesocellular siliceous foam (Mag-S-MCF). Then, chitosan was adsorbed onto the surface of Mag-S-MCF with pre-adsorbed enzymes, and further crosslinked via the glutaraldehyde treatment. The resulting composite materials, the crosslinked chitosan coating on Mag-S-MCF with pre-adsorbed CA (ADS-CA/CS-GA), could effectively prevent leaching of enzymes, showing no decrease of enzyme activity under shaking (200rpm) for 85days. ADS-CA/CS-GA also showed no activity decrease under recycled uses via facile magnetic separation, and could be successfully used for the biocatalytic CO2 conversion to bicarbonate, which was further utilized to generate calcium carbonate in the second batch reactor.",

keywords = "Calcium carbonate, Carbon conversion and utilization, Carbonic anhydrase, Crosslinked chitosan coating, Enzyme immobilization and stabilization, Magnetic mesoporous silica",

author = "Woo, {Kie Moon} and Inseon Lee and Hong, {Sung Gil} and Sunhyung An and Jinwoo Lee and Euichaul Oh and Jungbae Kim",

year = "2015",

month = sep,

day = "5",

doi = "10.1016/j.cej.2015.04.057",

language = "English",

volume = "276",

pages = "232--239",

journal = "Chemical Engineering Journal",

issn = "1385-8947",

publisher = "Elsevier",

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TY - JOUR

T1 - Crosslinked chitosan coating on magnetic mesoporous silica with pre-adsorbed carbonic anhydrase for carbon dioxide conversion

AU - Woo, Kie Moon

AU - Lee, Inseon

AU - Hong, Sung Gil

AU - An, Sunhyung

AU - Lee, Jinwoo

AU - Oh, Euichaul

AU - Kim, Jungbae

PY - 2015/9/5

Y1 - 2015/9/5

N2 - Carbonic anhydrase (CA), an enzyme converting CO2 to bicarbonate, was adsorbed into magnetically-separable spherical mesocellular siliceous foam (Mag-S-MCF). Then, chitosan was adsorbed onto the surface of Mag-S-MCF with pre-adsorbed enzymes, and further crosslinked via the glutaraldehyde treatment. The resulting composite materials, the crosslinked chitosan coating on Mag-S-MCF with pre-adsorbed CA (ADS-CA/CS-GA), could effectively prevent leaching of enzymes, showing no decrease of enzyme activity under shaking (200rpm) for 85days. ADS-CA/CS-GA also showed no activity decrease under recycled uses via facile magnetic separation, and could be successfully used for the biocatalytic CO2 conversion to bicarbonate, which was further utilized to generate calcium carbonate in the second batch reactor.

AB - Carbonic anhydrase (CA), an enzyme converting CO2 to bicarbonate, was adsorbed into magnetically-separable spherical mesocellular siliceous foam (Mag-S-MCF). Then, chitosan was adsorbed onto the surface of Mag-S-MCF with pre-adsorbed enzymes, and further crosslinked via the glutaraldehyde treatment. The resulting composite materials, the crosslinked chitosan coating on Mag-S-MCF with pre-adsorbed CA (ADS-CA/CS-GA), could effectively prevent leaching of enzymes, showing no decrease of enzyme activity under shaking (200rpm) for 85days. ADS-CA/CS-GA also showed no activity decrease under recycled uses via facile magnetic separation, and could be successfully used for the biocatalytic CO2 conversion to bicarbonate, which was further utilized to generate calcium carbonate in the second batch reactor.

KW - Calcium carbonate

KW - Carbon conversion and utilization

KW - Carbonic anhydrase

KW - Crosslinked chitosan coating

KW - Enzyme immobilization and stabilization

KW - Magnetic mesoporous silica

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