Crosslinked chitosan coating on magnetic mesoporous silica with pre-adsorbed carbonic anhydrase for carbon dioxide conversion

Kie Moon Woo, Inseon Lee, Sung Gil Hong, Sunhyung An, Jinwoo Lee, Euichaul Oh, Jungbae Kim

Research output: Contribution to journalArticle

10 Citations (Scopus)


Carbonic anhydrase (CA), an enzyme converting CO<inf>2</inf> to bicarbonate, was adsorbed into magnetically-separable spherical mesocellular siliceous foam (Mag-S-MCF). Then, chitosan was adsorbed onto the surface of Mag-S-MCF with pre-adsorbed enzymes, and further crosslinked via the glutaraldehyde treatment. The resulting composite materials, the crosslinked chitosan coating on Mag-S-MCF with pre-adsorbed CA (ADS-CA/CS-GA), could effectively prevent leaching of enzymes, showing no decrease of enzyme activity under shaking (200rpm) for 85days. ADS-CA/CS-GA also showed no activity decrease under recycled uses via facile magnetic separation, and could be successfully used for the biocatalytic CO<inf>2</inf> conversion to bicarbonate, which was further utilized to generate calcium carbonate in the second batch reactor.

Original languageEnglish
Pages (from-to)232-239
Number of pages8
JournalChemical Engineering Journal
Publication statusPublished - 2015 Sep 5



  • Calcium carbonate
  • Carbon conversion and utilization
  • Carbonic anhydrase
  • Crosslinked chitosan coating
  • Enzyme immobilization and stabilization
  • Magnetic mesoporous silica

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Chemistry(all)
  • Industrial and Manufacturing Engineering
  • Environmental Chemistry

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