TY - JOUR
T1 - Crosslinked chitosan coating on magnetic mesoporous silica with pre-adsorbed carbonic anhydrase for carbon dioxide conversion
AU - Woo, Kie Moon
AU - Lee, Inseon
AU - Hong, Sung Gil
AU - An, Sunhyung
AU - Lee, Jinwoo
AU - Oh, Euichaul
AU - Kim, Jungbae
N1 - Funding Information:
This work was supported by the ‘ International Collaborative R&D Program ’ (No. 20118510020020 ) and ‘ Energy Efficiency & Resources Core Technology Program ’ (No. 20142020200980 ) of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) Grants, funded by the Korea government Ministry of Trade, Industry & Energy . This work was also supported by the Global Research Laboratory Program ( 2014K1A1A2043032 ), and by the Nano·Material Technology Development Program ( 2014M3A7B4052193 ), all of which are funded by the Korea government Ministry of Science, ICT & Future Planning .
PY - 2015/9/5
Y1 - 2015/9/5
N2 - Carbonic anhydrase (CA), an enzyme converting CO2 to bicarbonate, was adsorbed into magnetically-separable spherical mesocellular siliceous foam (Mag-S-MCF). Then, chitosan was adsorbed onto the surface of Mag-S-MCF with pre-adsorbed enzymes, and further crosslinked via the glutaraldehyde treatment. The resulting composite materials, the crosslinked chitosan coating on Mag-S-MCF with pre-adsorbed CA (ADS-CA/CS-GA), could effectively prevent leaching of enzymes, showing no decrease of enzyme activity under shaking (200rpm) for 85days. ADS-CA/CS-GA also showed no activity decrease under recycled uses via facile magnetic separation, and could be successfully used for the biocatalytic CO2 conversion to bicarbonate, which was further utilized to generate calcium carbonate in the second batch reactor.
AB - Carbonic anhydrase (CA), an enzyme converting CO2 to bicarbonate, was adsorbed into magnetically-separable spherical mesocellular siliceous foam (Mag-S-MCF). Then, chitosan was adsorbed onto the surface of Mag-S-MCF with pre-adsorbed enzymes, and further crosslinked via the glutaraldehyde treatment. The resulting composite materials, the crosslinked chitosan coating on Mag-S-MCF with pre-adsorbed CA (ADS-CA/CS-GA), could effectively prevent leaching of enzymes, showing no decrease of enzyme activity under shaking (200rpm) for 85days. ADS-CA/CS-GA also showed no activity decrease under recycled uses via facile magnetic separation, and could be successfully used for the biocatalytic CO2 conversion to bicarbonate, which was further utilized to generate calcium carbonate in the second batch reactor.
KW - Calcium carbonate
KW - Carbon conversion and utilization
KW - Carbonic anhydrase
KW - Crosslinked chitosan coating
KW - Enzyme immobilization and stabilization
KW - Magnetic mesoporous silica
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U2 - 10.1016/j.cej.2015.04.057
DO - 10.1016/j.cej.2015.04.057
M3 - Article
AN - SCOPUS:84929497643
VL - 276
SP - 232
EP - 239
JO - Chemical Engineering Journal
JF - Chemical Engineering Journal
SN - 1385-8947
ER -