Cryopreserved Human Oocytes and Cord Blood Cells Can Produce Somatic Cell Nuclear Transfer-Derived Pluripotent Stem Cells with a Homozygous HLA Type

Jeoung Eun Lee, Ji Yoon Lee, Chang Hwan Park, Jin Hee Eum, Soo Kyung Jung, A. Reum Han, Dong Won Seol, Jin Saem Lee, Hyun Soo Shin, Jung Ho Im, Taehoon Chun, Kyungsoo Ha, Deok Rim Heo, Tae Ki Yoon, Dong Ryul Lee

Research output: Contribution to journalArticle

Abstract

Human pluripotent stem cells (PSCs) through somatic cell nuclear transfer (SCNT) may be an important source for regenerative medicine. The low derivation efficiency of stem cells and the accessibility of human oocytes are the main obstacles to their application. We previously reported that the efficiency of SCNT was increased by overexpression of H3K9me3 demethylase. Here, we applied a modified derivation method to the PSC line and first obtained human SCNT-PSC lines derived from both donated cryopreserved oocytes and cord blood cells with a homozygous human leukocyte antigen (HLA) type. The SCNT-PSCs have very similar characteristics with embryonic stem cells (ESCs) and additionally have shown immunocompatibility in an in vitro and in vivo humanized mouse with a matching HLA type. Our study demonstrates that SCNT technology using donated cryopreserved oocytes and cord blood cells with a known HLA type provides a promising method for establishing a human HLA-matched SCNT-PSC bank for regenerative medicine. In this article, Lee and his colleagues reported the successful production of HLA-homozygous SCNT-PSCs using MNCs from frozen/thawed donated cord blood in a public bank and frozen (vitrified) human oocytes donated after storage for 5 years. Homozygous SCNT-PSC-derived cells are highly immunocompatible with allogeneic HLA-matched immune cells. Therefore, we may suggest that this SCNT technology facilitates the establishment of the human HLA-matched SCNT-PSC bank.

Original languageEnglish
Pages (from-to)171-184
Number of pages14
JournalStem Cell Reports
Volume15
Issue number1
DOIs
Publication statusPublished - 2020 Jul 14

Keywords

  • frozen cord blood cells
  • frozen oocytes
  • homozygous HLA
  • pluripotent stem cells
  • somatic cell nuclear transfer

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Developmental Biology
  • Cell Biology

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  • Cite this

    Lee, J. E., Lee, J. Y., Park, C. H., Eum, J. H., Jung, S. K., Han, A. R., Seol, D. W., Lee, J. S., Shin, H. S., Im, J. H., Chun, T., Ha, K., Heo, D. R., Yoon, T. K., & Lee, D. R. (2020). Cryopreserved Human Oocytes and Cord Blood Cells Can Produce Somatic Cell Nuclear Transfer-Derived Pluripotent Stem Cells with a Homozygous HLA Type. Stem Cell Reports, 15(1), 171-184. https://doi.org/10.1016/j.stemcr.2020.05.005