Crystal structure of an EfPDF complex with Met-Ala-Ser based on crystallographic packing

Ki Hyun Nam, Kook Han Kim, Eunice Eun Kyeong Kim, Kwang Yeon Hwang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

PDF (peptide deformylase) plays a critical role in the production of mature proteins by removing the N-formyl polypeptide of nascent proteins in the prokaryote cell system. This protein is essential for bacterial growth, making it an attractive target for the design of new antibiotics. Accordingly, PDF has been evaluated as a drug target; however, architectural mechanism studies of PDF have not yet fully elucidated its molecular function. We recently reported the crystal structure of PDF produced by Enterococcus faecium [K.H. Nam, J.I. Ham, A. Priyadarshi, E.E. Kim, N. Chung, K.Y. Hwang, "Insight into the antibacterial drug design and architectural mechanism of peptide recognition from the E. faecium peptide deformylase structure", Proteins 74 (2009) 261-265]. Here, we present the crystal structure of the EfPDF complex with MAS (Met-Ser-Ala), thereby not only delineating the architectural mechanism for the recognition of mimic-peptides by N-terminal cleaved expression peptide, but also suggesting possible targets for rational design of antibacterial drugs. In addition to their implications for drug design, these structural studies will facilitate elucidation of the architectural mechanism responsible for the peptide recognition of PDF.

Original languageEnglish
Pages (from-to)630-633
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume381
Issue number4
DOIs
Publication statusPublished - 2009 Apr 17

Fingerprint

peptide deformylase
methionyl-alanyl-serine
Crystal structure
Drug Design
Peptides
Pharmaceutical Preparations
Proteins
Enterococcus faecium

Keywords

  • Antibacterial drug design
  • EfPDF-MAS
  • PDF
  • Peptide deformylase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Crystal structure of an EfPDF complex with Met-Ala-Ser based on crystallographic packing. / Nam, Ki Hyun; Kim, Kook Han; Kim, Eunice Eun Kyeong; Hwang, Kwang Yeon.

In: Biochemical and Biophysical Research Communications, Vol. 381, No. 4, 17.04.2009, p. 630-633.

Research output: Contribution to journalArticle

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