Crystal structure of an intracellular protease from Pyrococcus horikoshii at 2-Å resolution

Xinlin Du, In-Geol Choi, Rosalind Kim, Weiru Wang, Jaru Jancarik, Hisao Yokota, Sung Hou Kim

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90 Citations (Scopus)


The intracellular protease from Pyrococcus horikoshii (PH1704) and Pfpl from Pyrococcus furiosus are members of a class of intracellular proteases that have no sequence homology to any other known protease family. We report the crystal structure of PH1704 at 2.0-Å resolution. The protease is tentatively identified as a cysteine protease based on the presence of cysteine (residue 100) in a nucleophile elbow motif. In the crystal, PH1704 forms a hexameric ring structure, and the active sites are formed at the interfaces between three pairs of monomers.

Original languageEnglish
Pages (from-to)14079-14084
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number26
Publication statusPublished - 2000 Dec 19
Externally publishedYes


ASJC Scopus subject areas

  • Genetics
  • General

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