Crystal structure of bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase from Thermoplasma acidophilum

Won Ho Lee, Min Woo Sung, Jae Hee Kim, Young Kwan Kim, Arum Han, Kwang Yeon Hwang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Folate co-enzymes play a pivotal role in one-carbon transfer cellular processes. Many eukaryotes encode the tri-functional tetrahydrofolate dehydrogenase/cyclohydrolase/synthetase (deh/cyc/syn) enzyme, which consists of a N-terminal bifunctional domain (deh/cyc) and a C-terminal monofunctional domain (syn). Here, we report the first analogous archeal enzyme structures, for the bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase from Thermoplasma acidophilum (TaMTHFDC) as the native protein and also as its NADP complex. The TaMTHFDC structure is a dimer with a polar interface, as well as a NADP binding site that shows minor conformational change. The orientations of the residues in the NADP binding site do not change on ligand binding, incorporating three water molecules which are hydrogen bonded with phosphate groups of NADP in the structure of the complex. Our structural information will contribute to an improved understanding of the basis of THF and one-carbon metabolism.

Original languageEnglish
Pages (from-to)459-463
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume406
Issue number3
DOIs
Publication statusPublished - 2011 Mar 18

Fingerprint

Methylenetetrahydrofolate Dehydrogenase (NADP)
Thermoplasma
NADP
Crystal structure
Enzymes
Carbon
Binding Sites
Tetrahydrofolate Dehydrogenase
Ligases
Eukaryota
Folic Acid
Metabolism
Dimers
Hydrogen
Phosphates
Ligands
Molecules
Water
Proteins

Keywords

  • 5,10-Methylenetetrahydrofolate dehydrogenase/cyclohydrolase
  • Folate
  • NADP complex structure
  • One-carbon metabolism
  • Tetrahydrofolate pathway

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Crystal structure of bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase from Thermoplasma acidophilum. / Lee, Won Ho; Sung, Min Woo; Kim, Jae Hee; Kim, Young Kwan; Han, Arum; Hwang, Kwang Yeon.

In: Biochemical and Biophysical Research Communications, Vol. 406, No. 3, 18.03.2011, p. 459-463.

Research output: Contribution to journalArticle

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AU - Hwang, Kwang Yeon

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