Crystal structure of DeSI-1, a novel deSUMOylase belonging to a putative isopeptidase superfamily

Hye Young Suh, Ji Hoon Kim, Jae Sung Woo, Bonsu Ku, Eun Ju Shin, Yungdae Yun, Byung Ha Oh

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Post-translational modification by small ubiquitin-like modifier (SUMO) can be reversed by sentrin/SUMO-specific proteases (SENPs), the first known class of deSUMOylase. Recently, we identified a new deSUMOylating enzyme DeSI-1, which is distinct from SENPs and belongs to the putative deubiquitinating isopeptidase PPPDE superfamily. Herein, we report the crystal structure of DeSI-1, revealing that this enzyme forms a homodimer and that the groove between the two subunits is the active site harboring two absolutely conserved cysteine and histidine residues that form a catalytic dyad. We also show that DeSI-1 exhibits an extremely low endopeptidase activity toward precursor forms of SUMO-1 and SUMO-2, unlike SENPs.

Original languageEnglish
Pages (from-to)2099-2104
Number of pages6
JournalProteins: Structure, Function and Bioinformatics
Volume80
Issue number8
DOIs
Publication statusPublished - 2012 Aug 1
Externally publishedYes

Fingerprint

SUMO-1 Protein
Ubiquitin
Crystal structure
Peptide Hydrolases
Endopeptidases
Enzymes
Post Translational Protein Processing
Histidine
Cysteine
Catalytic Domain
isopeptidase

Keywords

  • DeSI-1
  • DeSUMOylase
  • PPPDE
  • Structure
  • SUMO
  • Ubiquitin-like protein

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology

Cite this

Crystal structure of DeSI-1, a novel deSUMOylase belonging to a putative isopeptidase superfamily. / Suh, Hye Young; Kim, Ji Hoon; Woo, Jae Sung; Ku, Bonsu; Shin, Eun Ju; Yun, Yungdae; Oh, Byung Ha.

In: Proteins: Structure, Function and Bioinformatics, Vol. 80, No. 8, 01.08.2012, p. 2099-2104.

Research output: Contribution to journalArticle

Suh, Hye Young ; Kim, Ji Hoon ; Woo, Jae Sung ; Ku, Bonsu ; Shin, Eun Ju ; Yun, Yungdae ; Oh, Byung Ha. / Crystal structure of DeSI-1, a novel deSUMOylase belonging to a putative isopeptidase superfamily. In: Proteins: Structure, Function and Bioinformatics. 2012 ; Vol. 80, No. 8. pp. 2099-2104.
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