Crystal structure of Legionella pneumophila type IV secretion system effector LegAS4

Jonghyeon Son, Chang Hwa Jo, Ravichandran N. Murugan, Jeong Kyu Bang, Kwang Yeon Hwang, Woo Cheol Lee

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The SET domain of LegAS4, a type IV secretion system effector of Legionella pneumophila, is a eukaryotic protein motif involved in histone methylation and epigenetic modulation. The SET domain of LegAS4 is involved in the modification of Lys4 of histone H3 (H3K4) in the nucleolus of the host cell, thereby enhancing heterochromatic rDNA transcription. Moreover, LegAS4 contains an ankyrin repeat domain of unknown function at its C-terminal region. Here, we report the crystal structure of LegAS4 in complex with S-adenosyl-l-methionine (SAM). Our data indicate that the ankyrin repeats interact extensively with the SET domain, especially with the SAM-binding amino acids, through conserved residues. Conserved surface analysis marks Glu159, Glu203, and Glu206 on the SET domain serve as candidate residues involved in interaction with the positively charged histone tail. Conserved surface residues on the ankyrin repeat domain surround a small pocket, which is suspected to serve as a binding site for an unknown ligand.

Original languageEnglish
Pages (from-to)817-824
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume465
Issue number4
DOIs
Publication statusPublished - 2015 Aug 16

Fingerprint

Ankyrin Repeat
Legionella pneumophila
Histones
Crystal structure
Methionine
Cell Nucleolus
Amino Acid Motifs
Methylation
Surface analysis
Transcription
Ribosomal DNA
Epigenomics
Binding Sites
Modulation
Ligands
Amino Acids
Type IV Secretion Systems
Proteins

Keywords

  • Ankyrin repeat
  • LegAS4
  • Legionella pneumophila
  • RomA
  • SET domain

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Crystal structure of Legionella pneumophila type IV secretion system effector LegAS4. / Son, Jonghyeon; Jo, Chang Hwa; Murugan, Ravichandran N.; Bang, Jeong Kyu; Hwang, Kwang Yeon; Lee, Woo Cheol.

In: Biochemical and Biophysical Research Communications, Vol. 465, No. 4, 16.08.2015, p. 817-824.

Research output: Contribution to journalArticle

Son, Jonghyeon ; Jo, Chang Hwa ; Murugan, Ravichandran N. ; Bang, Jeong Kyu ; Hwang, Kwang Yeon ; Lee, Woo Cheol. / Crystal structure of Legionella pneumophila type IV secretion system effector LegAS4. In: Biochemical and Biophysical Research Communications. 2015 ; Vol. 465, No. 4. pp. 817-824.
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