Crystal structure of Pyrococcus furiosus PF2050, a member of the DUF2666 protein family

Byeong Gu Han, Kyung Chae Jeong, Jea Won Cho, Byung Cheon Jeong, Hyun Kyu Song, Jae Young Lee, Dong Hyuk Shin, Sangho Lee, Byung Il Lee

Research output: Contribution to journalArticle

Abstract

Pyrococcus furiosus PF2050 is an uncharacterized putative protein that contains two DUF2666 domains. Functional and structural studies of PF2050 have not previously been performed. In this study, we determined the crystal structure of PF2050. The structure of PF2050 showed that the two DUF2666 domains interact tightly, forming a globular structure. Each DUF2666 domain comprises an antiparallel β-sheet and an α-helical bundle. One side of the PF2050 structure has a positively charged basic cleft, which may have a DNA-binding function. Furthermore, we confirmed that PF2050 interacts with circular and linear dsDNA.

Original languageEnglish
Pages (from-to)1384-1388
Number of pages5
JournalFEBS Letters
Volume586
Issue number9
DOIs
Publication statusPublished - 2012 May 7

Fingerprint

Pyrococcus furiosus
Crystal structure
DNA
Proteins

Keywords

  • DNA binding
  • DUF2666
  • PF2050
  • Pyrococcus furiosus

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Han, B. G., Jeong, K. C., Cho, J. W., Jeong, B. C., Song, H. K., Lee, J. Y., ... Lee, B. I. (2012). Crystal structure of Pyrococcus furiosus PF2050, a member of the DUF2666 protein family. FEBS Letters, 586(9), 1384-1388. https://doi.org/10.1016/j.febslet.2012.04.004

Crystal structure of Pyrococcus furiosus PF2050, a member of the DUF2666 protein family. / Han, Byeong Gu; Jeong, Kyung Chae; Cho, Jea Won; Jeong, Byung Cheon; Song, Hyun Kyu; Lee, Jae Young; Shin, Dong Hyuk; Lee, Sangho; Lee, Byung Il.

In: FEBS Letters, Vol. 586, No. 9, 07.05.2012, p. 1384-1388.

Research output: Contribution to journalArticle

Han, BG, Jeong, KC, Cho, JW, Jeong, BC, Song, HK, Lee, JY, Shin, DH, Lee, S & Lee, BI 2012, 'Crystal structure of Pyrococcus furiosus PF2050, a member of the DUF2666 protein family', FEBS Letters, vol. 586, no. 9, pp. 1384-1388. https://doi.org/10.1016/j.febslet.2012.04.004
Han, Byeong Gu ; Jeong, Kyung Chae ; Cho, Jea Won ; Jeong, Byung Cheon ; Song, Hyun Kyu ; Lee, Jae Young ; Shin, Dong Hyuk ; Lee, Sangho ; Lee, Byung Il. / Crystal structure of Pyrococcus furiosus PF2050, a member of the DUF2666 protein family. In: FEBS Letters. 2012 ; Vol. 586, No. 9. pp. 1384-1388.
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