Crystal structure of the periplasmic region of MacB, a noncanonic ABC transporter

Yongbin Xu, Se Hoon Sim, Hyun Nam Ki, Ling Jin Xiao, Hong Man Kim, Kwang Yeon Hwang, Kangseok Lee, Nam Chul Ha

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

MacB is a noncanonic ABC-type transporter within Gram-negative bacteria, which is responsible both for the efflux of macrolide antibiotics and for the secretion of heat-stable enterotoxin II. In Escherichia coli, MacB requires the membrane fusion protein MacA and the multifunctional outer membrane channel TolC to pump substrates to the external medium. Sequence analysis of MacB suggested that MacB has a relatively large periplasmic region. To gain insight into how MacB assembles with MacA and TolC, we determined the crystal structure of the periplasmic region of Actinobacillus actinomycetemcomitans MacB. Fold matching program reveals that parts of the MacB periplasmic region have structural motifs in common with the RND-type transporter AcrB. Since it behaved as a monomer in solution, our finding is consistent with the dimeric nature of full-length MacB, providing an insight into the assembly in the tripartite efflux pump.

Original languageEnglish
Pages (from-to)5218-5225
Number of pages8
JournalBiochemistry
Volume48
Issue number23
DOIs
Publication statusPublished - 2009 Jun 16

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Lepidium
ATP-Binding Cassette Transporters
Membrane Fusion Proteins
Crystal structure
Pumps
Aggregatibacter actinomycetemcomitans
Macrolides
Gram-Negative Bacteria
Ion Channels
Escherichia coli
Sequence Analysis
Bacteria
Monomers
Anti-Bacterial Agents
Substrates

ASJC Scopus subject areas

  • Biochemistry

Cite this

Xu, Y., Sim, S. H., Ki, H. N., Xiao, L. J., Kim, H. M., Hwang, K. Y., ... Ha, N. C. (2009). Crystal structure of the periplasmic region of MacB, a noncanonic ABC transporter. Biochemistry, 48(23), 5218-5225. https://doi.org/10.1021/bi900415t

Crystal structure of the periplasmic region of MacB, a noncanonic ABC transporter. / Xu, Yongbin; Sim, Se Hoon; Ki, Hyun Nam; Xiao, Ling Jin; Kim, Hong Man; Hwang, Kwang Yeon; Lee, Kangseok; Ha, Nam Chul.

In: Biochemistry, Vol. 48, No. 23, 16.06.2009, p. 5218-5225.

Research output: Contribution to journalArticle

Xu, Y, Sim, SH, Ki, HN, Xiao, LJ, Kim, HM, Hwang, KY, Lee, K & Ha, NC 2009, 'Crystal structure of the periplasmic region of MacB, a noncanonic ABC transporter', Biochemistry, vol. 48, no. 23, pp. 5218-5225. https://doi.org/10.1021/bi900415t
Xu, Yongbin ; Sim, Se Hoon ; Ki, Hyun Nam ; Xiao, Ling Jin ; Kim, Hong Man ; Hwang, Kwang Yeon ; Lee, Kangseok ; Ha, Nam Chul. / Crystal structure of the periplasmic region of MacB, a noncanonic ABC transporter. In: Biochemistry. 2009 ; Vol. 48, No. 23. pp. 5218-5225.
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AB - MacB is a noncanonic ABC-type transporter within Gram-negative bacteria, which is responsible both for the efflux of macrolide antibiotics and for the secretion of heat-stable enterotoxin II. In Escherichia coli, MacB requires the membrane fusion protein MacA and the multifunctional outer membrane channel TolC to pump substrates to the external medium. Sequence analysis of MacB suggested that MacB has a relatively large periplasmic region. To gain insight into how MacB assembles with MacA and TolC, we determined the crystal structure of the periplasmic region of Actinobacillus actinomycetemcomitans MacB. Fold matching program reveals that parts of the MacB periplasmic region have structural motifs in common with the RND-type transporter AcrB. Since it behaved as a monomer in solution, our finding is consistent with the dimeric nature of full-length MacB, providing an insight into the assembly in the tripartite efflux pump.

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