Crystal structure of transglutaminase 2 with GTP complex and amino acid sequence evidence of evolution of GTP binding site

Tae Ho Jang; Dong Sup Lee; Kihang Choi; Eui Man Jeong; In Gyu Kim; Young Whan Kim; Jung Nyeo Chun; Ju Hong Jeon; Hyun Ho Park

doi:10.1371/journal.pone.0107005

Crystal structure of transglutaminase 2 with GTP complex and amino acid sequence evidence of evolution of GTP binding site

Tae Ho Jang, Dong Sup Lee, Kihang Choi, Eui Man Jeong, In Gyu Kim, Young Whan Kim, Jung Nyeo Chun, Ju Hong Jeon, Hyun Ho Park

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

Transglutaminase2 (TG2) is a multi-functional protein involved in various cellular processes, including apoptosis, differentiation, wound healing, and angiogenesis. The malfunction of TG2 causes many human disease including inflammatory disease, celiac disease, neurodegenerative diseases, tissue fibrosis, and cancers. Protein cross-linking activity, which is representative of TG2, is activated by calcium ions and suppressed by GTP. Here, we elucidated the structure of TG2 in complex with its endogenous inhibitor, GTP. Our structure showed why GTP is the optimal nucleotide for interacting with and inhibiting TG2. In addition, sequence comparison provided information describing the evolutionary scenario of GTP usage for controlling the activity of TG2. Copyright

Original language	English
Article number	e107005
Journal	PloS one
Volume	9
Issue number	9
DOIs	https://doi.org/10.1371/journal.pone.0107005
Publication status	Published - 2014 Sep 5

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Crystal structure of transglutaminase 2 with GTP complex and amino acid sequence evidence of evolution of GTP binding site. / Jang, Tae Ho; Lee, Dong Sup; Choi, Kihang; Jeong, Eui Man; Kim, In Gyu; Kim, Young Whan; Chun, Jung Nyeo; Jeon, Ju Hong; Park, Hyun Ho.

In: PloS one, Vol. 9, No. 9, e107005, 05.09.2014.

Research output: Contribution to journal › Article › peer-review

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AU - Jeon, Ju Hong

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Crystal structure of transglutaminase 2 with GTP complex and amino acid sequence evidence of evolution of GTP binding site

Abstract

ASJC Scopus subject areas

UN SDGs

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