Crystal structures of Dronpa complexed with quenchable metal ions provide insight into metal biosensor development

In Jung Kim, Sangsoo Kim, Jeahyun Park, Intae Eom, Sunam Kim, Jin Hong Kim, Sung Chul Ha, Yeon Gil Kim, Kwang Yeon Hwang, Ki Hyun Nam

Research output: Contribution to journalLetter

6 Citations (Scopus)

Abstract

Many fluorescent proteins (FPs) show fluorescence quenching by specific metal ions, which can be applied towards metal biosensor development. In this study, we investigated the significant fluorescence quenching of Dronpa by Co2+ and Cu2+ ions. Crystal structures of Co2+-, Ni2+- and Cu2+ -bound Dronpa revealed previously unseen, unique, metal-binding sites for fluorescence quenching. These metal ions commonly interact with surface-exposed histidine residues (His194-His210 and His210-His212), and interact indirectly with chromophores. Structural analysis of the Co2+- and Cu2+- binding sites of Dronpa provides insight into FP-based metal biosensor engineering.

Original languageEnglish
Pages (from-to)2982-2990
Number of pages9
JournalFEBS Letters
DOIs
Publication statusPublished - 2016 Sep 1

Keywords

  • biosensor
  • Dronpa
  • fluorescent protein
  • metal-binding site
  • quenching

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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  • Cite this

    Kim, I. J., Kim, S., Park, J., Eom, I., Kim, S., Kim, J. H., Ha, S. C., Kim, Y. G., Hwang, K. Y., & Nam, K. H. (2016). Crystal structures of Dronpa complexed with quenchable metal ions provide insight into metal biosensor development. FEBS Letters, 2982-2990. https://doi.org/10.1002/1873-3468.12316