Crystal structures of enoyl-ACP reductases i (FabI) and III (FabL) from B. subtilis

Kook Han Kim, Byung Hak Ha, Su Jin Kim, Seung Kon Hong, Kwang Yeon Hwang, Eunice Eunkyeong Kim

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Enoyl-[acyl carrier protein] (ACP) reductase (ENR) is a key enzyme in type II fatty acid synthesis that catalyzes the last step in each elongation cycle. Therefore, it has been considered as a target for antibiotics. However, recent studies indicate that some pathogens have more than one ENR; in particular, Bacillus subtilis has two ENRs, FabI and FabL. The crystal structures of the ternary complexes of BsFaBI and BsFabL are found as a homotetramer showing the same overall structure despite a sequence identity of only 24%. The positions of the catalytic dyad of Tyr-(Xaa)6-Lys in FabL are almost identical to that of FabI, but a detailed structural analysis shows that FabL shares more structural similarities with FabG and other members of the SDR (short-chain alcohol dehydrogenase/reductase) family. The apo FabL structure shows significantly different conformations at the cofactor and the substrate-binding regions, and this resulted in a totally different tetrameric arrangement reflecting the flexibility of these regions in the absence of the cofactor and substrate/inhibitor.

Original languageEnglish
Pages (from-to)403-415
Number of pages13
JournalJournal of Molecular Biology
Volume406
Issue number3
DOIs
Publication statusPublished - 2011 Feb 25

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Acyl Carrier Protein
Oxidoreductases
Alcohol Dehydrogenase
Bacillus subtilis
Fatty Acids
Anti-Bacterial Agents
Enzymes

Keywords

  • crystal structure
  • enoyl-ACP reductase
  • FabI
  • FabL
  • fatty acid biosynthesis

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Crystal structures of enoyl-ACP reductases i (FabI) and III (FabL) from B. subtilis. / Kim, Kook Han; Ha, Byung Hak; Kim, Su Jin; Hong, Seung Kon; Hwang, Kwang Yeon; Kim, Eunice Eunkyeong.

In: Journal of Molecular Biology, Vol. 406, No. 3, 25.02.2011, p. 403-415.

Research output: Contribution to journalArticle

Kim, Kook Han ; Ha, Byung Hak ; Kim, Su Jin ; Hong, Seung Kon ; Hwang, Kwang Yeon ; Kim, Eunice Eunkyeong. / Crystal structures of enoyl-ACP reductases i (FabI) and III (FabL) from B. subtilis. In: Journal of Molecular Biology. 2011 ; Vol. 406, No. 3. pp. 403-415.
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