Crystallization and preliminary X-ray analysis of a complex between the Bowman-Birk trypsin inhibitor from barley and porcine pancreatic trypsin

Young Sil Kim, Hyun Kyu Song, Se Won Suh

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A 1:2 complex between the Bowman-Birk trypsin inhibitor from barley seeds and porcine pancreatic trypsin has been crystallized at 291 K using polyethylene glycol as precipitant. The crystals belong to the orthorhombic space group P212121, with unit-cell parameters a = 67.10, b = 88.38 and c = 203.65 Å. The asymmetric unit contains two monomers of the complex, with a corresponding V(m) of 2.41 293 Da-1 and a solvent content of 49%. Native data to 2.2 Å resolution have been collected at 100 K using synchrotron X-rays.

Original languageEnglish
Pages (from-to)1244-1246
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume55
Issue number6
DOIs
Publication statusPublished - 1999 Jun
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology

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