Crystallization and preliminary X-ray analysis of glutamate racemase from Aquifex pyrophilus, a hyperthermophilic bacterium

Kwang Yeon Hwang; Chun Seok Cho; Sang Suk Kim; Kyuwon Baek; Sung Hou Kim; Yeon Gyu Yu; Yunje Cho

doi:10.1107/S0907444999000608

Crystallization and preliminary X-ray analysis of glutamate racemase from Aquifex pyrophilus, a hyperthermophilic bacterium

Kwang Yeon Hwang, Chun Seok Cho, Sang Suk Kim, Kyuwon Baek, Sung Hou Kim, Yeon Gyu Yu, Yunje Cho

Department of Biosystems and Biotechnology

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Glutamate racemase catalyzes the reversible reaction of L.-glutamate to D-glutamate, an essential component of the bacterial cell wall. Glutamate racemase from Aquifex pyrophilus has been crystallized by the hanging-drop vapor-diffusion method using polyethylene glycol 6000 as a precipitant. The crystals belong to space group P6₁22 or P6₅22 with unit-cell parameters a = b = 72.1, c = 185.02 Å. The asymmetric unit contains one molecule, corresponding to a V(m) value of 2.35 Å³ Da^-1. Complete data sets from a native and a mercury-derivative crystal have been collected at 2.0 and 2.3 Å resolution, respectively, using a synchrotron-radiation source.

Original language	English
Pages (from-to)	927-928
Number of pages	2
Journal	Acta Crystallographica Section D: Biological Crystallography
Volume	55
Issue number	4
DOIs	https://doi.org/10.1107/S0907444999000608
Publication status	Published - 1999 Apr

ASJC Scopus subject areas

Structural Biology

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Crystallization and preliminary X-ray analysis of glutamate racemase from Aquifex pyrophilus, a hyperthermophilic bacterium. / Hwang, Kwang Yeon; Cho, Chun Seok; Kim, Sang Suk; Baek, Kyuwon; Kim, Sung Hou; Yu, Yeon Gyu; Cho, Yunje.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 55, No. 4, 04.1999, p. 927-928.

Research output: Contribution to journal › Article › peer-review

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abstract = "Glutamate racemase catalyzes the reversible reaction of L.-glutamate to D-glutamate, an essential component of the bacterial cell wall. Glutamate racemase from Aquifex pyrophilus has been crystallized by the hanging-drop vapor-diffusion method using polyethylene glycol 6000 as a precipitant. The crystals belong to space group P6122 or P6522 with unit-cell parameters a = b = 72.1, c = 185.02 {\AA}. The asymmetric unit contains one molecule, corresponding to a V(m) value of 2.35 {\AA}3 Da-1. Complete data sets from a native and a mercury-derivative crystal have been collected at 2.0 and 2.3 {\AA} resolution, respectively, using a synchrotron-radiation source.",

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Crystallization and preliminary X-ray analysis of glutamate racemase from Aquifex pyrophilus, a hyperthermophilic bacterium

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