Crystallization and preliminary X-ray analysis of glutamate racemase from Aquifex pyrophilus, a hyperthermophilic bacterium

Kwang Yeon Hwang, Chun Seok Cho, Sang Suk Kim, Kyuwon Baek, Sung Hou Kim, Yeon Gyu Yu, Yunje Cho

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Glutamate racemase catalyzes the reversible reaction of L.-glutamate to D-glutamate, an essential component of the bacterial cell wall. Glutamate racemase from Aquifex pyrophilus has been crystallized by the hanging-drop vapor-diffusion method using polyethylene glycol 6000 as a precipitant. The crystals belong to space group P6122 or P6522 with unit-cell parameters a = b = 72.1, c = 185.02 Å. The asymmetric unit contains one molecule, corresponding to a V(m) value of 2.35 Å3 Da-1. Complete data sets from a native and a mercury-derivative crystal have been collected at 2.0 and 2.3 Å resolution, respectively, using a synchrotron-radiation source.

Original languageEnglish
Pages (from-to)927-928
Number of pages2
JournalActa Crystallographica Section D: Biological Crystallography
Volume55
Issue number4
DOIs
Publication statusPublished - 1999 Apr 1
Externally publishedYes

Fingerprint

glutamate racemase
glutamates
X ray analysis
Crystallization
bacteria
Glutamic Acid
Bacteria
X-Rays
crystallization
Crystals
Synchrotrons
Synchrotron radiation
Mercury
Cell Wall
x rays
Vapors
Cells
Radiation
Derivatives
Molecules

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Condensed Matter Physics
  • Structural Biology

Cite this

Crystallization and preliminary X-ray analysis of glutamate racemase from Aquifex pyrophilus, a hyperthermophilic bacterium. / Hwang, Kwang Yeon; Cho, Chun Seok; Kim, Sang Suk; Baek, Kyuwon; Kim, Sung Hou; Yu, Yeon Gyu; Cho, Yunje.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 55, No. 4, 01.04.1999, p. 927-928.

Research output: Contribution to journalArticle

Hwang, Kwang Yeon ; Cho, Chun Seok ; Kim, Sang Suk ; Baek, Kyuwon ; Kim, Sung Hou ; Yu, Yeon Gyu ; Cho, Yunje. / Crystallization and preliminary X-ray analysis of glutamate racemase from Aquifex pyrophilus, a hyperthermophilic bacterium. In: Acta Crystallographica Section D: Biological Crystallography. 1999 ; Vol. 55, No. 4. pp. 927-928.
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