TY - JOUR
T1 - Crystallization and preliminary X-ray analysis of glutamate racemase from Aquifex pyrophilus, a hyperthermophilic bacterium
AU - Hwang, Kwang Yeon
AU - Cho, Chun Seok
AU - Kim, Sang Suk
AU - Baek, Kyuwon
AU - Kim, Sung Hou
AU - Yu, Yeon Gyu
AU - Cho, Yunje
PY - 1999/4
Y1 - 1999/4
N2 - Glutamate racemase catalyzes the reversible reaction of L.-glutamate to D-glutamate, an essential component of the bacterial cell wall. Glutamate racemase from Aquifex pyrophilus has been crystallized by the hanging-drop vapor-diffusion method using polyethylene glycol 6000 as a precipitant. The crystals belong to space group P6122 or P6522 with unit-cell parameters a = b = 72.1, c = 185.02 Å. The asymmetric unit contains one molecule, corresponding to a V(m) value of 2.35 Å3 Da-1. Complete data sets from a native and a mercury-derivative crystal have been collected at 2.0 and 2.3 Å resolution, respectively, using a synchrotron-radiation source.
AB - Glutamate racemase catalyzes the reversible reaction of L.-glutamate to D-glutamate, an essential component of the bacterial cell wall. Glutamate racemase from Aquifex pyrophilus has been crystallized by the hanging-drop vapor-diffusion method using polyethylene glycol 6000 as a precipitant. The crystals belong to space group P6122 or P6522 with unit-cell parameters a = b = 72.1, c = 185.02 Å. The asymmetric unit contains one molecule, corresponding to a V(m) value of 2.35 Å3 Da-1. Complete data sets from a native and a mercury-derivative crystal have been collected at 2.0 and 2.3 Å resolution, respectively, using a synchrotron-radiation source.
UR - http://www.scopus.com/inward/record.url?scp=0033119154&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033119154&partnerID=8YFLogxK
U2 - 10.1107/S0907444999000608
DO - 10.1107/S0907444999000608
M3 - Article
C2 - 10089337
AN - SCOPUS:0033119154
VL - 55
SP - 927
EP - 928
JO - Acta Crystallographica Section D: Structural Biology
JF - Acta Crystallographica Section D: Structural Biology
SN - 0907-4449
IS - 4
ER -