Crystallization and preliminary X-ray analysis of Saccharomyces cerevisiae Ypd1p, a key intermediate in phosphorelay signal transduction

Myong Gyong Lee, Jae Young Lee, Hyun Kyu Song, Se Won Suh

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Ypd1p, a 167-residue protein from Saccharomyces cerevisiae, plays a key role in osmosensing phosphorelay signal transduction. It forms part of a multistep phosphorelay system which also includes Sln1p hybrid histidine kinase and two response regulators, Ssk1p and Skn7p. It has been overexpressed in soluble form in Escherichia coli with a His 6-tag at its C-terminus. The recombinant protein has been crystallized at room temperature using ammonium sulfate and lithium sulfate as precipitants. Native diffraction data have been collected to 2.3 Å using synchrotron radiation. The crystals are triclinic, belonging to the space group P1, with unit-cell parameters a = 65.78, b = 66.74, c = 65.75 Å, α = 106.60, β = 106.48, γ = 115.53°. The asymmetric unit contains four molecules of the monomeric recombinant Ypd1p, with a corresponding V(m) of 2.75 Å 3 Da -1 and a solvent content of 55.3%.

Original languageEnglish
Pages (from-to)1219-1221
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume55
Issue number6
DOIs
Publication statusPublished - 1999 Jun 1
Externally publishedYes

Fingerprint

His-His-His-His-His-His
Saccharomyces cerevisiae Proteins
saccharomyces
Signal transduction
Synchrotrons
X ray analysis
Ammonium Sulfate
Crystallization
Synchrotron radiation
Recombinant Proteins
Histidine
Yeast
Escherichia coli
Saccharomyces cerevisiae
Signal Transduction
Phosphotransferases
Diffraction
X-Rays
lithium sulfates
Radiation

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Condensed Matter Physics
  • Structural Biology

Cite this

Crystallization and preliminary X-ray analysis of Saccharomyces cerevisiae Ypd1p, a key intermediate in phosphorelay signal transduction. / Lee, Myong Gyong; Lee, Jae Young; Song, Hyun Kyu; Suh, Se Won.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 55, No. 6, 01.06.1999, p. 1219-1221.

Research output: Contribution to journalArticle

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