Crystallization and preliminary X-ray analysis of the C-terminal fragment of Ski7 from Saccharomyces cerevisiae

Ji Young Lee, Si Hoon Park, Byung Cheon Jeong, Hyun Kyu Song

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Ski7 (superkiller protein 7) plays a critical role in the mRNA surveillance pathway. The C-terminal fragment of Ski7 (residues 520-747) from Saccharomyces cerevisiae was heterologously expressed in Escherichia coli and purified to homogeneity. It was successfully crystallized and preliminary X-ray data were collected to 2.0Å resolution using synchrotron radiation. The crystal belonged to a trigonal space group, either P3121 or P3221, with unit-cell parameters a = b = 73.5, c = 83.6Å. The asymmetric unit contains one molecule of the C-terminal fragment of Ski7 with a corresponding crystal volume per protein mass (V M) of 2.61Å3Da-1 and a solvent content of 52.8% by volume. The merging R factor is 6.6%. Structure determination by MAD phasing is under way.

Original languageEnglish
Pages (from-to)1252-1255
Number of pages4
JournalActa Crystallographica Section:F Structural Biology Communications
Volume70
DOIs
Publication statusPublished - 2014 Sep 1

Keywords

  • RNA degradation
  • Saccharomyces cerevisiae
  • Ski7
  • mRNA surveillance
  • nonstop decay

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

Fingerprint

Dive into the research topics of 'Crystallization and preliminary X-ray analysis of the C-terminal fragment of Ski7 from Saccharomyces cerevisiae'. Together they form a unique fingerprint.

Cite this