Crystallization and preliminary X-ray crystallographic analysis of human nucleoside diphosphate kinase A

Kyeongsik Min, Sun Young Kim, Hyun Kyu Song, Changsoo Chang, Seung Je Cho, Jinho Moon, Jin Kuk Yang, Jae Young Lee, Kong Joo Lee, Se Won Suh

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Human nucleoside diphosphate kinase A catalyzes phosphoryl transfer and acts as a suppressor of metastasis. It has been crystallized using 2-methyl-2,4-pentanediol as a precipitant at 288 K. The crystal is monoclinic, belonging to the space group P21, with unit-cell parameters a = 74.21, b = 78.11, c = 82.29 Å, β = 101.33°. The asymmetric unit contains a homohexamer, with a corresponding crystal volume per protein mass (V(m)) of 2.27 Å3 Da-1 and a solvent content of 46%. Native X-ray data to 2.15 Å resolution have been collected using synchrotron X-rays.

Original languageEnglish
Pages (from-to)504-505
Number of pages2
JournalActa Crystallographica Section D: Biological Crystallography
Volume56
Issue number4
DOIs
Publication statusPublished - 2000 Jan 1
Externally publishedYes

Fingerprint

diphosphates
nucleosides
Crystallization
X-Rays
crystallization
suppressors
X rays
Crystals
Synchrotrons
metastasis
crystals
synchrotrons
x rays
Neoplasm Metastasis
proteins
cells
Proteins
human NME1 protein
hexylene glycol

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Clinical Biochemistry
  • Structural Biology
  • Condensed Matter Physics

Cite this

Crystallization and preliminary X-ray crystallographic analysis of human nucleoside diphosphate kinase A. / Min, Kyeongsik; Kim, Sun Young; Song, Hyun Kyu; Chang, Changsoo; Cho, Seung Je; Moon, Jinho; Yang, Jin Kuk; Lee, Jae Young; Lee, Kong Joo; Suh, Se Won.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 56, No. 4, 01.01.2000, p. 504-505.

Research output: Contribution to journalArticle

Min, Kyeongsik ; Kim, Sun Young ; Song, Hyun Kyu ; Chang, Changsoo ; Cho, Seung Je ; Moon, Jinho ; Yang, Jin Kuk ; Lee, Jae Young ; Lee, Kong Joo ; Suh, Se Won. / Crystallization and preliminary X-ray crystallographic analysis of human nucleoside diphosphate kinase A. In: Acta Crystallographica Section D: Biological Crystallography. 2000 ; Vol. 56, No. 4. pp. 504-505.
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AU - Cho, Seung Je

AU - Moon, Jinho

AU - Yang, Jin Kuk

AU - Lee, Jae Young

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