CyaY is a 106-residue protein from Escherichia coli. It shows aminoacid sequence similarity to human frataxin and a frataxin homologue in Saccharomyces cerevisiae, Yfh1p. The former is associated with the disease Friedreich ataxia and the latter plays a key role in iron homeostasis in mitochondria. CyaY has been overexpressed in soluble form in E. coli. The recombinant protein with a His6 tag at its C-terminus has been crystallized at 296 K using polyethylene glycol (PEG) 4000 as a precipitant. Native diffraction data have been collected to 1.8 Å using Cu Kα X-rays. The crystals belong to the trigonal space group P3121 (or P3221), with unit-cell parameters a = b = 44.66, c = 99.87 Å, α = β = 90.0, γ= 120.0°. The asymmetric unit contains one molecule of recombinant CyaY, with a corresponding V(m) of 2.13 Å3 Da-1 and solvent content of 42.3%.
|Number of pages||2|
|Journal||Acta Crystallographica Section D: Biological Crystallography|
|Publication status||Published - 2000|
ASJC Scopus subject areas
- Structural Biology