Crystallization and preliminary X-ray crystallographic analysis of carboxylesterase from Pseudomonas fluorescens

K. K. Kim, Kwang Yeon Hwang, W. H. Jang, H. Lee, O. J. Yoo, M. U. Choi, S. W. Suh

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Large crystals of carboxylesterase from Pseudomonas fluorescens have been grown in the presence of dioxane using ammonium sulfate and lithium sulfate as precipitant. They belong to the tetragonal space group P41212 (or P43212), with unit cell dimensions of a = 82.01 (±0.06) Å and c = 145.44 (±0.08) Å. The presence of one carboxylesterase dimer in the asymmetric unit gives the crystal volume per protein mass (V(M)) of 2.56 Å3/Da and solvent fraction of 52.0% by volume. The crystals diffract to at least 2.3 Å Bragg spacing when exposed to CuKα X rays from a rotating anode generator. X-ray data (nearly complete to 2.6 Å Bragg spacing) have been collected from a native crystal.

Original languageEnglish
Pages (from-to)417-419
Number of pages3
JournalArchives of Biochemistry and Biophysics
Volume302
Issue number2
DOIs
Publication statusPublished - 1993 Jan 1
Externally publishedYes

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Pseudomonas fluorescens
Carboxylesterase
Crystallization
X-Rays
X rays
Crystals
Ammonium Sulfate
Electrodes
Dimers
Anodes
Proteins
1,4-dioxane
lithium sulfate

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Crystallization and preliminary X-ray crystallographic analysis of carboxylesterase from Pseudomonas fluorescens. / Kim, K. K.; Hwang, Kwang Yeon; Jang, W. H.; Lee, H.; Yoo, O. J.; Choi, M. U.; Suh, S. W.

In: Archives of Biochemistry and Biophysics, Vol. 302, No. 2, 01.01.1993, p. 417-419.

Research output: Contribution to journalArticle

Kim, K. K. ; Hwang, Kwang Yeon ; Jang, W. H. ; Lee, H. ; Yoo, O. J. ; Choi, M. U. ; Suh, S. W. / Crystallization and preliminary X-ray crystallographic analysis of carboxylesterase from Pseudomonas fluorescens. In: Archives of Biochemistry and Biophysics. 1993 ; Vol. 302, No. 2. pp. 417-419.
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AU - Yoo, O. J.

AU - Choi, M. U.

AU - Suh, S. W.

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