Crystallization and preliminary X-ray crystallographic analysis of lipase from Pseudomonas cepacia

Kyeong Kyu Kim, Kwang Yeon Hwang, Hye Sung Jeon, Sangsoo Kim, Robert M. Sweet, Chul Hak Yang, Se Won Suh

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Large crystals of lipase from Pseudomonas cepacia have been grown at room temperature from solutions containing 2-methyl-2,4-pentanediol and sodium citrate. They grow within two weeks to typical dimensions of 1.0 mm × 0.5 mm × 0.3 mm. The crystals belong to the monoclinic space group P21, with unit cell parameters a=84.91 Å, b=47.33 Å, c=86.00 Å, and β=116.09°. And they diffract to about 1.6 Å upon exposure to synchroton X-rays. X-ray data have been collected to 2.2 Å Bragg spacing from a native crystal.

Original languageEnglish
Pages (from-to)1258-1262
Number of pages5
JournalJournal of Molecular Biology
Volume227
Issue number4
Publication statusPublished - 1992 Dec 1
Externally publishedYes

Fingerprint

Burkholderia cepacia
Crystallization
Lipase
X-Rays
Temperature
sodium citrate
hexylene glycol

Keywords

  • Crystallization
  • Lipase
  • Preliminary X-ray analysis
  • Pseudomonas cepacia
  • X-ray crystallography

ASJC Scopus subject areas

  • Virology

Cite this

Kim, K. K., Hwang, K. Y., Jeon, H. S., Kim, S., Sweet, R. M., Yang, C. H., & Suh, S. W. (1992). Crystallization and preliminary X-ray crystallographic analysis of lipase from Pseudomonas cepacia. Journal of Molecular Biology, 227(4), 1258-1262.

Crystallization and preliminary X-ray crystallographic analysis of lipase from Pseudomonas cepacia. / Kim, Kyeong Kyu; Hwang, Kwang Yeon; Jeon, Hye Sung; Kim, Sangsoo; Sweet, Robert M.; Yang, Chul Hak; Suh, Se Won.

In: Journal of Molecular Biology, Vol. 227, No. 4, 01.12.1992, p. 1258-1262.

Research output: Contribution to journalArticle

Kim, KK, Hwang, KY, Jeon, HS, Kim, S, Sweet, RM, Yang, CH & Suh, SW 1992, 'Crystallization and preliminary X-ray crystallographic analysis of lipase from Pseudomonas cepacia', Journal of Molecular Biology, vol. 227, no. 4, pp. 1258-1262.
Kim, Kyeong Kyu ; Hwang, Kwang Yeon ; Jeon, Hye Sung ; Kim, Sangsoo ; Sweet, Robert M. ; Yang, Chul Hak ; Suh, Se Won. / Crystallization and preliminary X-ray crystallographic analysis of lipase from Pseudomonas cepacia. In: Journal of Molecular Biology. 1992 ; Vol. 227, No. 4. pp. 1258-1262.
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