Crystallization and preliminary X-ray crystallographic analysis of α-amylase from Bacillus subtilis

Changsoo Chang, Kyeong Kyu Kim, Kwang Yeon Hwang, Myung Un Choi, Se Won Suh

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Large crystals of α-amylaae from Bacillus subtilis have been obtained at room temperature using polyethylene glycol 6000 as precipitant. They grow to typical dimensions of 0.25 mm × 0.3 mm × 2.0 mm in five days. The crystals belong to the orthorhombic space group P212121, with unit cell dimensions of a = 85.46 Å, b = 166.5 Å and c = 332.7 Å. The asymmetric unit seems to contain eight molecules of α-amylase, with crystal volume per protein mass (Vm) of 2.69 Å3/Da and solvent content of 54.3% by volume. Despite a very long c-axis, the crystals diffracted to about 2.2 Å Bragg spacing using the rotating anode X-rays and were resistant to damage by X-rays. Thus they are suitable for structure determination by X-ray methods at high resolution. X-ray diffraction data have been collected to 3.4 Å Bragg spacing from a native crystal.

Original languageEnglish
Pages (from-to)235-238
Number of pages4
JournalJournal of Molecular Biology
Volume229
Issue number1
Publication statusPublished - 1993 Dec 1
Externally publishedYes

Fingerprint

Amylases
Crystallization
Bacillus subtilis
X-Rays
X-Ray Diffraction
Electrodes
Temperature
Proteins

Keywords

  • α-amylase
  • Bacillus subtilis
  • Crystallization
  • Preliminary X-ray analysis
  • X-ray crystallography

ASJC Scopus subject areas

  • Virology

Cite this

Crystallization and preliminary X-ray crystallographic analysis of α-amylase from Bacillus subtilis. / Chang, Changsoo; Kim, Kyeong Kyu; Hwang, Kwang Yeon; Choi, Myung Un; Suh, Se Won.

In: Journal of Molecular Biology, Vol. 229, No. 1, 01.12.1993, p. 235-238.

Research output: Contribution to journalArticle

Chang, Changsoo ; Kim, Kyeong Kyu ; Hwang, Kwang Yeon ; Choi, Myung Un ; Suh, Se Won. / Crystallization and preliminary X-ray crystallographic analysis of α-amylase from Bacillus subtilis. In: Journal of Molecular Biology. 1993 ; Vol. 229, No. 1. pp. 235-238.
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