Crystallization and preliminary X-ray crystallographic analysis of arylesterase from Pseudomonas fluorescens

Kyu Kim Kyeong Kyu Kim, Kwang Yeon Hwang, Duk Choi Kang Duk Choi, Hyun Kang Joo Hyun Kang, Joon Yoo Ook Joon Yoo, Won Suh Se Won Suh

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Large crystals of arylesterase from Pseudomonas fluorescens have been grown at room temperature using ammonium sulfate as a precipitant. They grow to dimensions of 0.7 x 0.7 x 0.6 mm3 within a month. The crystals belong to the trigonal space group P31 (or P32), with unit cell dimensions of a = 147.12 Å and c = 131.08 Å. The asymmetric unit seems to contain six molecules of dimeric arylesterase, with corresponding crystal volume per protein mass (V(M)) of 2.53 Å3/Da and solvent fraction of 51.5% by volume. The crystals diffract to at least 2.2 Å Bragg spacing when exposed to X- rays from a rotating-anode source. X-ray data have been collected to 2.9 Å Bragg spacing from native crystals.

Original languageEnglish
Pages (from-to)213-215
Number of pages3
JournalProteins: Structure, Function and Genetics
Volume15
Issue number2
DOIs
Publication statusPublished - 1993 Feb 17
Externally publishedYes

Fingerprint

Pseudomonas fluorescens
Crystallization
X-Rays
X rays
Crystals
Ammonium Sulfate
Electrodes
Temperature
Proteins
Anodes
arylesterase
Molecules

Keywords

  • bacterial esterase
  • crystals
  • X-ray diffraction

ASJC Scopus subject areas

  • Genetics
  • Structural Biology
  • Biochemistry

Cite this

Kyeong Kyu Kim, K. K., Hwang, K. Y., Kang Duk Choi, D. C., Joo Hyun Kang, H. K., Ook Joon Yoo, J. Y., & Se Won Suh, W. S. (1993). Crystallization and preliminary X-ray crystallographic analysis of arylesterase from Pseudomonas fluorescens. Proteins: Structure, Function and Genetics, 15(2), 213-215. https://doi.org/10.1002/prot.340150212

Crystallization and preliminary X-ray crystallographic analysis of arylesterase from Pseudomonas fluorescens. / Kyeong Kyu Kim, Kyu Kim; Hwang, Kwang Yeon; Kang Duk Choi, Duk Choi; Joo Hyun Kang, Hyun Kang; Ook Joon Yoo, Joon Yoo; Se Won Suh, Won Suh.

In: Proteins: Structure, Function and Genetics, Vol. 15, No. 2, 17.02.1993, p. 213-215.

Research output: Contribution to journalArticle

Kyeong Kyu Kim, KK, Hwang, KY, Kang Duk Choi, DC, Joo Hyun Kang, HK, Ook Joon Yoo, JY & Se Won Suh, WS 1993, 'Crystallization and preliminary X-ray crystallographic analysis of arylesterase from Pseudomonas fluorescens', Proteins: Structure, Function and Genetics, vol. 15, no. 2, pp. 213-215. https://doi.org/10.1002/prot.340150212
Kyeong Kyu Kim, Kyu Kim ; Hwang, Kwang Yeon ; Kang Duk Choi, Duk Choi ; Joo Hyun Kang, Hyun Kang ; Ook Joon Yoo, Joon Yoo ; Se Won Suh, Won Suh. / Crystallization and preliminary X-ray crystallographic analysis of arylesterase from Pseudomonas fluorescens. In: Proteins: Structure, Function and Genetics. 1993 ; Vol. 15, No. 2. pp. 213-215.
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