Crystallization and preliminary X-ray crystallographic analysis of the protease inhibitor ecotin in complex with chymotrypsin

Cheol Soon Lee, Ihn Sik Seong, Hyun Kyu Song, Chin Ha Chung, Se Won Suh

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Ecotin, a homodimeric protein composed of 142-residue subunits, is a novel protease inhibitor present in the periplasm of Escherichia coli. It shows a broad inhibitory specificity towards a group of serine proteases and binds two molecules of protease to form a tetrameric complex in a distinct chelation mechanism. The ecotin-chymotrypsin complex has been crystallized in the triclinic space group P1 with unit-cell parameters a = 57.29, b = 57.39, c = 79.75 Å, α = 91.49, β = 88.63 and γ = 112.45°. The asymmetric unit contains the whole tetrameric complex, consisting of two molecules of chymotrypsin bound to the ecotin dimer, with a corresponding crystal volume per protein mass (V(M)) of 2.58 Å 3 Da -1 and a solvent fraction of 48.9%. The crystals diffract beyond 2.0 Å with Cu Kα X-rays and are very stable in the X-ray beam. Native X-ray data have been collected from a crystal to approximately 2.0 Å Bragg spacing.

Original languageEnglish
Pages (from-to)1091-1092
Number of pages2
JournalActa Crystallographica Section D: Biological Crystallography
Volume55
Issue number5
DOIs
Publication statusPublished - 1999 May 1
Externally publishedYes

Fingerprint

protease
Chymotrypsin
Crystallization
Protease Inhibitors
inhibitors
X-Rays
crystallization
X rays
Crystals
crystals
proteins
Periplasm
chelation
Molecules
x rays
Serine Proteases
Escherichia
Chelation
Dimers
Escherichia coli

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Condensed Matter Physics
  • Structural Biology

Cite this

Crystallization and preliminary X-ray crystallographic analysis of the protease inhibitor ecotin in complex with chymotrypsin. / Lee, Cheol Soon; Seong, Ihn Sik; Song, Hyun Kyu; Chung, Chin Ha; Suh, Se Won.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 55, No. 5, 01.05.1999, p. 1091-1092.

Research output: Contribution to journalArticle

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