Crystallization and preliminary X-ray crystallographic analysis of the tRNA-specific adenosine deaminase from Streptococcus pyogenes

Min J. Ku, Won H. Lee, Ki Hyun Nam, Kyeong H. Rhee, Ki Seog Lee, Eunice Eunkyung Kim, Myung H. Yu, Kwang Yeon Hwang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The tRNA-specific adenosine deaminase from the pathogenic bacteria Streptococcus pyogenes (spTAD) has been overexpressed in Escherichia coli and crystallized in the presence of Zn2+ ion at 295 K using ammonium sulfate as a precipitant. Flash-cooled crystals of spTAD diffracted to 2.0 Å using 30%(v/v) glycerol as a cryoprotectant. X-ray diffraction data have been collected to 2.0 Å using synchrotron radiation. The crystal belongs to the tetragonal space group P42212, with unit-cell parameters a = b = 81.042, c = 81.270 Å. The asymmetric unit contains one subunit of spTAD, with a corresponding crystal volume per protein weight (V M) of 3.3 Å3 Da-1 and a solvent content of 62.7%.

Original languageEnglish
Pages (from-to)375-377
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume61
Issue number4
DOIs
Publication statusPublished - 2005 Dec 1
Externally publishedYes

Fingerprint

streptococcus
adenosines
Synchrotrons
Adenosine Deaminase
Streptococcus pyogenes
Ammonium Sulfate
Transfer RNA
Crystallization
X-Ray Diffraction
Glycerol
X-Rays
Ions
Radiation
crystallization
Escherichia coli
Bacteria
Weights and Measures
X rays
Crystals
crystals

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Structural Biology
  • Condensed Matter Physics

Cite this

Crystallization and preliminary X-ray crystallographic analysis of the tRNA-specific adenosine deaminase from Streptococcus pyogenes. / Ku, Min J.; Lee, Won H.; Nam, Ki Hyun; Rhee, Kyeong H.; Lee, Ki Seog; Kim, Eunice Eunkyung; Yu, Myung H.; Hwang, Kwang Yeon.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 61, No. 4, 01.12.2005, p. 375-377.

Research output: Contribution to journalArticle

@article{ad49bb60ad49447292235dcea7041063,
title = "Crystallization and preliminary X-ray crystallographic analysis of the tRNA-specific adenosine deaminase from Streptococcus pyogenes",
abstract = "The tRNA-specific adenosine deaminase from the pathogenic bacteria Streptococcus pyogenes (spTAD) has been overexpressed in Escherichia coli and crystallized in the presence of Zn2+ ion at 295 K using ammonium sulfate as a precipitant. Flash-cooled crystals of spTAD diffracted to 2.0 {\AA} using 30{\%}(v/v) glycerol as a cryoprotectant. X-ray diffraction data have been collected to 2.0 {\AA} using synchrotron radiation. The crystal belongs to the tetragonal space group P42212, with unit-cell parameters a = b = 81.042, c = 81.270 {\AA}. The asymmetric unit contains one subunit of spTAD, with a corresponding crystal volume per protein weight (V M) of 3.3 {\AA}3 Da-1 and a solvent content of 62.7{\%}.",
author = "Ku, {Min J.} and Lee, {Won H.} and Nam, {Ki Hyun} and Rhee, {Kyeong H.} and Lee, {Ki Seog} and Kim, {Eunice Eunkyung} and Yu, {Myung H.} and Hwang, {Kwang Yeon}",
year = "2005",
month = "12",
day = "1",
doi = "10.1107/S1744309105007311",
language = "English",
volume = "61",
pages = "375--377",
journal = "Acta Crystallographica Section F:Structural Biology Communications",
issn = "1744-3091",
publisher = "John Wiley and Sons Ltd",
number = "4",

}

TY - JOUR

T1 - Crystallization and preliminary X-ray crystallographic analysis of the tRNA-specific adenosine deaminase from Streptococcus pyogenes

AU - Ku, Min J.

AU - Lee, Won H.

AU - Nam, Ki Hyun

AU - Rhee, Kyeong H.

AU - Lee, Ki Seog

AU - Kim, Eunice Eunkyung

AU - Yu, Myung H.

AU - Hwang, Kwang Yeon

PY - 2005/12/1

Y1 - 2005/12/1

N2 - The tRNA-specific adenosine deaminase from the pathogenic bacteria Streptococcus pyogenes (spTAD) has been overexpressed in Escherichia coli and crystallized in the presence of Zn2+ ion at 295 K using ammonium sulfate as a precipitant. Flash-cooled crystals of spTAD diffracted to 2.0 Å using 30%(v/v) glycerol as a cryoprotectant. X-ray diffraction data have been collected to 2.0 Å using synchrotron radiation. The crystal belongs to the tetragonal space group P42212, with unit-cell parameters a = b = 81.042, c = 81.270 Å. The asymmetric unit contains one subunit of spTAD, with a corresponding crystal volume per protein weight (V M) of 3.3 Å3 Da-1 and a solvent content of 62.7%.

AB - The tRNA-specific adenosine deaminase from the pathogenic bacteria Streptococcus pyogenes (spTAD) has been overexpressed in Escherichia coli and crystallized in the presence of Zn2+ ion at 295 K using ammonium sulfate as a precipitant. Flash-cooled crystals of spTAD diffracted to 2.0 Å using 30%(v/v) glycerol as a cryoprotectant. X-ray diffraction data have been collected to 2.0 Å using synchrotron radiation. The crystal belongs to the tetragonal space group P42212, with unit-cell parameters a = b = 81.042, c = 81.270 Å. The asymmetric unit contains one subunit of spTAD, with a corresponding crystal volume per protein weight (V M) of 3.3 Å3 Da-1 and a solvent content of 62.7%.

UR - http://www.scopus.com/inward/record.url?scp=33744499464&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33744499464&partnerID=8YFLogxK

U2 - 10.1107/S1744309105007311

DO - 10.1107/S1744309105007311

M3 - Article

C2 - 16511045

AN - SCOPUS:33744499464

VL - 61

SP - 375

EP - 377

JO - Acta Crystallographica Section F:Structural Biology Communications

JF - Acta Crystallographica Section F:Structural Biology Communications

SN - 1744-3091

IS - 4

ER -