Crystallization and preliminary X-ray crystallographic analysis of probable amylase/protease inhibitor-B from rice seeds

Kwang Yeon Hwang, Kyeong Kyu Kim, Kyeongsik Min, Soo Hyun Eom, Yeon Gyu Yu, Sangsoo Kim, Robert M. Sweet, Se Won Suh

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Large crystals of probable amylase/protease inhibitor-B have been grown at room temperature from ammonium sulfate solution. The crystals grow within five days to dimensions of 0.6 mm x 0.6 mm x 0.6 mm. They diffract to at least 1.7 Å upon exposure to synchrotron X-rays. The crystals belong to the space group P41212 (or P43212) with unit cell dimensions of a = 38.02 Å and c = 98.98 Å. The presence of one molecule per asymmetric unit gives the unit cell volume per protein mass (Vm) of 1.99 Å3/Da and the solvent fraction of 38.2% by volume. X-ray data have been collected to 2.0 Å Bragg spacing from native crystals.

Original languageEnglish
Pages (from-to)255-257
Number of pages3
JournalJournal of Molecular Biology
Volume229
Issue number1
Publication statusPublished - 1993 Dec 1
Externally publishedYes

Fingerprint

Amylases
Crystallization
Protease Inhibitors
Seeds
X-Rays
Synchrotrons
Ammonium Sulfate
Cell Size
Temperature
Proteins
Oryza

Keywords

  • Crystallization
  • Phospholipid transfer protein
  • Possible amylase/protease inhibitor
  • Preliminary X-ray analysis
  • Rice seeds

ASJC Scopus subject areas

  • Virology

Cite this

Crystallization and preliminary X-ray crystallographic analysis of probable amylase/protease inhibitor-B from rice seeds. / Hwang, Kwang Yeon; Kim, Kyeong Kyu; Min, Kyeongsik; Eom, Soo Hyun; Yu, Yeon Gyu; Kim, Sangsoo; Sweet, Robert M.; Suh, Se Won.

In: Journal of Molecular Biology, Vol. 229, No. 1, 01.12.1993, p. 255-257.

Research output: Contribution to journalArticle

Hwang, KY, Kim, KK, Min, K, Eom, SH, Yu, YG, Kim, S, Sweet, RM & Suh, SW 1993, 'Crystallization and preliminary X-ray crystallographic analysis of probable amylase/protease inhibitor-B from rice seeds', Journal of Molecular Biology, vol. 229, no. 1, pp. 255-257.
Hwang, Kwang Yeon ; Kim, Kyeong Kyu ; Min, Kyeongsik ; Eom, Soo Hyun ; Yu, Yeon Gyu ; Kim, Sangsoo ; Sweet, Robert M. ; Suh, Se Won. / Crystallization and preliminary X-ray crystallographic analysis of probable amylase/protease inhibitor-B from rice seeds. In: Journal of Molecular Biology. 1993 ; Vol. 229, No. 1. pp. 255-257.
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AU - Kim, Sangsoo

AU - Sweet, Robert M.

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