Crystallization and preliminary X-ray crystallographic analysis of the protease inhibitor ecotin

Dong Hae Shin, Kwang Yeon Hwang, Kyeong Kyu Kim, Hae Ryun Lee, Cheol Soon Lee, Chin Ha Chung, Se Won Suh

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Ecotin, a novel serine protease inhibitor isolated from Escherichia coli, has been crystallized using polyethylene glycol 1500 as the precipitating agent. The crystals belong to the orthorhombic space group P212121, with unit cell parameters of a = 39.22 Å, b = 84.86 Å, and c = 98.74 Å. The asymmetric unit contains one dimeric molecule of ecotin, with a crystal volume per protein mass (Vm) of 2.55 Å3/Da and a solvent content of 51.8% by volume. The crystals diffract to at least 2.2 Å using a conventional X-ray source, and X-ray data have been collected to 2.7 Å Bragg spacing from a native crystal.

Original languageEnglish
Pages (from-to)1157-1158
Number of pages2
JournalJournal of Molecular Biology
Volume229
Issue number4
Publication statusPublished - 1993 Dec 1
Externally publishedYes

Fingerprint

Crystallization
Protease Inhibitors
X-Rays
Serine Proteinase Inhibitors
Escherichia coli
Proteins

Keywords

  • Crystallization
  • Ecotin
  • Preliminary X-ray analysis
  • Serine protease inhibitor
  • X-ray crystallography

ASJC Scopus subject areas

  • Virology

Cite this

Shin, D. H., Hwang, K. Y., Kim, K. K., Lee, H. R., Lee, C. S., Chung, C. H., & Suh, S. W. (1993). Crystallization and preliminary X-ray crystallographic analysis of the protease inhibitor ecotin. Journal of Molecular Biology, 229(4), 1157-1158.

Crystallization and preliminary X-ray crystallographic analysis of the protease inhibitor ecotin. / Shin, Dong Hae; Hwang, Kwang Yeon; Kim, Kyeong Kyu; Lee, Hae Ryun; Lee, Cheol Soon; Chung, Chin Ha; Suh, Se Won.

In: Journal of Molecular Biology, Vol. 229, No. 4, 01.12.1993, p. 1157-1158.

Research output: Contribution to journalArticle

Shin, DH, Hwang, KY, Kim, KK, Lee, HR, Lee, CS, Chung, CH & Suh, SW 1993, 'Crystallization and preliminary X-ray crystallographic analysis of the protease inhibitor ecotin', Journal of Molecular Biology, vol. 229, no. 4, pp. 1157-1158.
Shin, Dong Hae ; Hwang, Kwang Yeon ; Kim, Kyeong Kyu ; Lee, Hae Ryun ; Lee, Cheol Soon ; Chung, Chin Ha ; Suh, Se Won. / Crystallization and preliminary X-ray crystallographic analysis of the protease inhibitor ecotin. In: Journal of Molecular Biology. 1993 ; Vol. 229, No. 4. pp. 1157-1158.
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