Crystallization and preliminary X-ray crystallographic analysis of human nucleoside diphosphate kinase A.

K. Min, S. Y. Kim, H. K. Song, C. Chang, S. J. Cho, J. Moon, J. K. Yang, J. Y. Lee, K. J. Lee, S. W. Suh

Research output: Contribution to journalArticle

Abstract

Human nucleoside diphosphate kinase A catalyzes phosphoryl transfer and acts as a suppressor of metastasis. It has been crystallized using 2-methyl-2,4-pentanediol as a precipitant at 288 K. The crystal is monoclinic, belonging to the space group P2(1), with unit-cell parameters a = 74.21, b = 78.11, c = 82.29 A, beta = 101. 33 degrees. The asymmetric unit contains a homohexamer, with a corresponding crystal volume per protein mass (V(m)) of 2.27 A(3) Da(-1) and a solvent content of 46%. Native X-ray data to 2.15 A resolution have been collected using synchrotron X-rays.

Original languageEnglish
JournalActa crystallographica. Section D, Biological crystallography
Volume56
Publication statusPublished - 2000 Apr 1
Externally publishedYes

Fingerprint

diphosphates
nucleosides
Crystallization
X-Rays
crystallization
suppressors
X rays
Crystals
Synchrotrons
metastasis
crystals
synchrotrons
x rays
Neoplasm Metastasis
proteins
cells
Proteins
human NME1 protein
hexylene glycol

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Condensed Matter Physics
  • Structural Biology

Cite this

Crystallization and preliminary X-ray crystallographic analysis of human nucleoside diphosphate kinase A. / Min, K.; Kim, S. Y.; Song, H. K.; Chang, C.; Cho, S. J.; Moon, J.; Yang, J. K.; Lee, J. Y.; Lee, K. J.; Suh, S. W.

In: Acta crystallographica. Section D, Biological crystallography, Vol. 56, 01.04.2000.

Research output: Contribution to journalArticle

Min, K. ; Kim, S. Y. ; Song, H. K. ; Chang, C. ; Cho, S. J. ; Moon, J. ; Yang, J. K. ; Lee, J. Y. ; Lee, K. J. ; Suh, S. W. / Crystallization and preliminary X-ray crystallographic analysis of human nucleoside diphosphate kinase A. In: Acta crystallographica. Section D, Biological crystallography. 2000 ; Vol. 56.
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AU - Kim, S. Y.

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AU - Chang, C.

AU - Cho, S. J.

AU - Moon, J.

AU - Yang, J. K.

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AU - Suh, S. W.

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