Crystallization and preliminary X-ray crystallographic analysis of NAD+-dependent DNA ligase from Thermus filiformis

Jae Young Lee, Hyun Kyu Kim, Changsoo Chang, Soo Hyun Eom, Kwang Yeon Hwang, Yunje Cho, Yeon Gyu Yu, Seong Eon Ryu, Suk Tae Kwon, Se Won Suh

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A highly thermostable DNA ligase from Thermus filiformis has been crystallized at room temperature using methoxypolyethylene glycol 5000 as a precipitant. The crystal belongs to the monoclinic space group P21, with unit-cell parameters a = 90.63, b = 117.80, c = 98.65 Å, β = 115.56°. Two molecules of DNA ligase are present in the asymmetric unit, giving a crystal volume per protein mass (V(m)) of 3.1 Å3Da-1 and a solvent content of 61%. A native data set extending to 3.0 Å resolution has been collected at 100 K using synchrotron X-rays.

Original languageEnglish
Pages (from-to)357-358
Number of pages2
JournalActa Crystallographica Section D: Biological Crystallography
Volume56
Issue number3
DOIs
Publication statusPublished - 2000

ASJC Scopus subject areas

  • Structural Biology

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