Crystallization and preliminary X-ray crystallographic analysis of NAD+-dependent DNA ligase from Thermus filiformis

Jae Young Lee, Hyun Kyu Kim, Changsoo Chang, Soo Hyun Eom, Kwang Yeon Hwang, Yunje Cho, Yeon Gyu Yu, Seong Eon Ryu, Suk Tae Kwon, Se Won Suh

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A highly thermostable DNA ligase from Thermus filiformis has been crystallized at room temperature using methoxypolyethylene glycol 5000 as a precipitant. The crystal belongs to the monoclinic space group P21, with unit-cell parameters a = 90.63, b = 117.80, c = 98.65 Å, β = 115.56°. Two molecules of DNA ligase are present in the asymmetric unit, giving a crystal volume per protein mass (V(m)) of 3.1 Å3Da-1 and a solvent content of 61%. A native data set extending to 3.0 Å resolution has been collected at 100 K using synchrotron X-rays.

Original languageEnglish
Pages (from-to)357-358
Number of pages2
JournalActa Crystallographica Section D: Biological Crystallography
Volume56
Issue number3
DOIs
Publication statusPublished - 2000 Mar 25
Externally publishedYes

Fingerprint

Thermus
DNA Ligases
Crystallization
NAD
deoxyribonucleic acid
X-Rays
crystallization
X rays
Crystals
Synchrotrons
crystals
glycols
synchrotrons
x rays
proteins
Molecules
Temperature
room temperature
cells
molecules

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Condensed Matter Physics
  • Structural Biology

Cite this

Crystallization and preliminary X-ray crystallographic analysis of NAD+-dependent DNA ligase from Thermus filiformis. / Lee, Jae Young; Kim, Hyun Kyu; Chang, Changsoo; Eom, Soo Hyun; Hwang, Kwang Yeon; Cho, Yunje; Yu, Yeon Gyu; Ryu, Seong Eon; Kwon, Suk Tae; Suh, Se Won.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 56, No. 3, 25.03.2000, p. 357-358.

Research output: Contribution to journalArticle

Lee, Jae Young ; Kim, Hyun Kyu ; Chang, Changsoo ; Eom, Soo Hyun ; Hwang, Kwang Yeon ; Cho, Yunje ; Yu, Yeon Gyu ; Ryu, Seong Eon ; Kwon, Suk Tae ; Suh, Se Won. / Crystallization and preliminary X-ray crystallographic analysis of NAD+-dependent DNA ligase from Thermus filiformis. In: Acta Crystallographica Section D: Biological Crystallography. 2000 ; Vol. 56, No. 3. pp. 357-358.
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AU - Cho, Yunje

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