Crystallization and preliminary x-ray crystallographic studies of ketosteroid isomerase from Pseudomonas putida biotype B

Byung Ha Oh, Suhng Wook Kim, Seong Eon Ryu, Sang Soo Kim, Mi Kyung Yoon, Kwan Yong Choi

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

The Δ5-3-ketosteroid isomerase from Pseudomonas putida biotype B has been crystallized. The crystals belong to the space group P212121 with unit cell dimensions of a = 36.48 Å, b = 74.30 Å, c = 96.02 Å, and contain one homodimer per asymmetric unit. Native diffraction data to 2.19 Å resolution have been obtained from one crystal at room temperature indicating that the crystals are quite suitable for structure determination by multiple isomorphous replacement.

Original languageEnglish
Pages (from-to)514-515
Number of pages2
JournalProteins: Structure, Function and Genetics
Volume24
Issue number4
DOIs
Publication statusPublished - 1996
Externally publishedYes

Keywords

  • crystallization
  • ketosteroid isomerase
  • protein-steroid interaction

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology

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