Crystallization and preliminary x-ray crystallographic studies of ketosteroid isomerase from Pseudomonas putida biotype B

Byung Ha Oh, Suhng Wook Kim, Seong Eon Ryu, Sang Soo Kim, Mi Kyung Yoon, Kwan Yong Choi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The Δ 5 -3-ketosteroid isomerase from Pseudomonas putida biotype B has been crystallized. The crystals belong to the space group P2 1 2 1 2 1 with unit cell dimensions of a = 36.48 Å, b = 74.30 Å, c = 96.02 Å, and contain one homodimer per asymmetric unit. Native diffraction data to 2.19 Å resolution have been obtained from one crystal at room temperature indicating that the crystals are quite suitable for structure determination by multiple isomorphous replacement.

Original languageEnglish
Pages (from-to)514-515
Number of pages2
JournalProteins: Structure, Function and Genetics
Volume24
Issue number4
DOIs
Publication statusPublished - 1996 May 3
Externally publishedYes

Fingerprint

steroid delta-isomerase
Ketosteroids
Isomerases
Pseudomonas putida
Crystallization
X-Rays
X rays
Crystals
Temperature
Diffraction

Keywords

  • crystallization
  • ketosteroid isomerase
  • protein-steroid interaction

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology

Cite this

Crystallization and preliminary x-ray crystallographic studies of ketosteroid isomerase from Pseudomonas putida biotype B. / Oh, Byung Ha; Kim, Suhng Wook; Ryu, Seong Eon; Kim, Sang Soo; Yoon, Mi Kyung; Choi, Kwan Yong.

In: Proteins: Structure, Function and Genetics, Vol. 24, No. 4, 03.05.1996, p. 514-515.

Research output: Contribution to journalArticle

Oh, Byung Ha ; Kim, Suhng Wook ; Ryu, Seong Eon ; Kim, Sang Soo ; Yoon, Mi Kyung ; Choi, Kwan Yong. / Crystallization and preliminary x-ray crystallographic studies of ketosteroid isomerase from Pseudomonas putida biotype B. In: Proteins: Structure, Function and Genetics. 1996 ; Vol. 24, No. 4. pp. 514-515.
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