Crystallization and preliminary X-ray crystallographic studies of the N-terminal domain of human ribosomal protein L7a (RPL7a)

Tae Ho Jang, Jin Hee Park, Ju Hong Jeon, Dong Sup Lee, Kihang Choi, In Gyu Kim, Young Whan Kim, Hyun Ho Park

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Ribosomal proteins are a major component of ribosomes, which catalyze protein synthesis. One ribosomal protein, L7a (RPL7a), which is a component of the 60S large ribosomal subunit, has additional functions involved in cell growth and differentiation that occur via interaction with human thyroid hormone receptor (THR) and retinoic acid receptor (RAR) and in turn inhibit the activities of the two nuclear hormone receptors. In this study, the N-terminal domain of human RPL7a was overexpressed in Escherichia coli using an engineered C - terminal His tag. The N-terminal domain of human RPL7a was then purified to homogeneity and crystallized at 293 K. X-ray diffraction data were collected to a resolution of 3.5 Å from a crystal belonging to the tetragonal space group P4122 or P4322 with unit-cell parameters a = 92.28, b = 92.28, c = 236.59 Å.

Original languageEnglish
Pages (from-to)510-512
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume67
Issue number4
DOIs
Publication statusPublished - 2011 Apr 1

Fingerprint

Ribosomal Proteins
Crystallization
Eukaryotic Large Ribosome Subunits
Large Ribosome Subunits
X-Rays
crystallization
proteins
Thyroid Hormone Receptors
X rays
Retinoic Acid Receptors
hormones
Cell growth
Cytoplasmic and Nuclear Receptors
Ribosomes
X-Ray Diffraction
Escherichia coli
Cell Differentiation
x rays
protein synthesis
ribosomes

Keywords

  • ribosome
  • RPL7a

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Structural Biology
  • Genetics
  • Condensed Matter Physics

Cite this

Crystallization and preliminary X-ray crystallographic studies of the N-terminal domain of human ribosomal protein L7a (RPL7a). / Jang, Tae Ho; Park, Jin Hee; Jeon, Ju Hong; Lee, Dong Sup; Choi, Kihang; Kim, In Gyu; Kim, Young Whan; Park, Hyun Ho.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 67, No. 4, 01.04.2011, p. 510-512.

Research output: Contribution to journalArticle

Jang, Tae Ho ; Park, Jin Hee ; Jeon, Ju Hong ; Lee, Dong Sup ; Choi, Kihang ; Kim, In Gyu ; Kim, Young Whan ; Park, Hyun Ho. / Crystallization and preliminary X-ray crystallographic studies of the N-terminal domain of human ribosomal protein L7a (RPL7a). In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 2011 ; Vol. 67, No. 4. pp. 510-512.
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