TY - JOUR
T1 - Crystallization and preliminary X-ray crystallographic studies of glutamate racemase from Lactobacillus fermenti
AU - Lee, Ki Seog
AU - Park, Seon Mi
AU - Hwang, Kwang Yeon
AU - Chi, Young Min
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2005
Y1 - 2005
N2 - Glutamate racemase catalyzes the conversion of L-glutamic acid to D-glutamic acid and vice versa. Since D-glutamic acid is one of the essential amino acids present in peptidoglycan, glutamate racemase has been considered to be an attractive target for the design of new antibacterial drugs. Glutamate racemase from Lactobacillus fermenti has been crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol 8000 as a precipitant. The crystals belong to the orthorhombic space group C2221, with unit-cell parameters a = 98.32, b = 184.09, c = 45.99 Å. The asymmetric unit contains one molecule, corresponding to a VM value of 1.84 Å3 Da-1. A complete data set has been collected from the native enzyme at 2.28 Å resolution using a synchrotron-radiation source.
AB - Glutamate racemase catalyzes the conversion of L-glutamic acid to D-glutamic acid and vice versa. Since D-glutamic acid is one of the essential amino acids present in peptidoglycan, glutamate racemase has been considered to be an attractive target for the design of new antibacterial drugs. Glutamate racemase from Lactobacillus fermenti has been crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol 8000 as a precipitant. The crystals belong to the orthorhombic space group C2221, with unit-cell parameters a = 98.32, b = 184.09, c = 45.99 Å. The asymmetric unit contains one molecule, corresponding to a VM value of 1.84 Å3 Da-1. A complete data set has been collected from the native enzyme at 2.28 Å resolution using a synchrotron-radiation source.
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U2 - 10.1107/S1744309104034426
DO - 10.1107/S1744309104034426
M3 - Article
C2 - 16510993
AN - SCOPUS:33646478885
VL - 61
SP - 199
EP - 201
JO - Acta Crystallographica Section F:Structural Biology Communications
JF - Acta Crystallographica Section F:Structural Biology Communications
SN - 1744-3091
IS - 2
ER -