Abstract
Succinic semialdehyde dehydrogenase (SSADH) plays a critical role in the metabolism of the inhibitory neurotransmitter γ-aminobutyric acid (GABA) and catalyzes the NAD(P) +-coupled oxidation of succinic semialdehyde (SSA) to succinic acid (SA). SSADH from Streptococcus pyogenes has been purified and crystallized as the apoenzyme and in a complex with NAD +. The crystals of native and NAD +-complexed SSADH diffracted to resolutions of 1.6 and 1.7 Å, respectively, using a synchrotron-radiation source. Both crystals belonged to the orthorhombic space group P2 12 12 1, with unit-cell parameters a = 93.3, b = 100.3, c = 105.1 Å for the native crystal and a = 93.3, b = 100.3, c = 105.0 Å for the complex crystal. Preliminary molecular replacement confirmed the presence of one dimer in both crystals, corresponding to a Matthews coefficient (V M) of 2.37 Å 3 Da -1 and a solvent content of 48.0%.
| Original language | English |
|---|---|
| Pages (from-to) | 288-291 |
| Number of pages | 4 |
| Journal | Acta Crystallographica Section F: Structural Biology and Crystallization Communications |
| Volume | 68 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2012 Mar 1 |
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Keywords
- GabD
- NAD
- Streptococcus pyogenes
- succinic semialdehyde dehydrogenase
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Structural Biology
- Genetics
- Condensed Matter Physics
Cite this
Crystallization and preliminary X-ray crystallographic studies of succinic semialdehyde dehydrogenase from Streptococcus pyogenes. / Jang, Eun Hyuk; Lim, Jong Eun; Chi, Young Min; Lee, Ki Seog.
In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 68, No. 3, 01.03.2012, p. 288-291.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Crystallization and preliminary X-ray crystallographic studies of succinic semialdehyde dehydrogenase from Streptococcus pyogenes
AU - Jang, Eun Hyuk
AU - Lim, Jong Eun
AU - Chi, Young Min
AU - Lee, Ki Seog
PY - 2012/3/1
Y1 - 2012/3/1
N2 - Succinic semialdehyde dehydrogenase (SSADH) plays a critical role in the metabolism of the inhibitory neurotransmitter γ-aminobutyric acid (GABA) and catalyzes the NAD(P) +-coupled oxidation of succinic semialdehyde (SSA) to succinic acid (SA). SSADH from Streptococcus pyogenes has been purified and crystallized as the apoenzyme and in a complex with NAD +. The crystals of native and NAD +-complexed SSADH diffracted to resolutions of 1.6 and 1.7 Å, respectively, using a synchrotron-radiation source. Both crystals belonged to the orthorhombic space group P2 12 12 1, with unit-cell parameters a = 93.3, b = 100.3, c = 105.1 Å for the native crystal and a = 93.3, b = 100.3, c = 105.0 Å for the complex crystal. Preliminary molecular replacement confirmed the presence of one dimer in both crystals, corresponding to a Matthews coefficient (V M) of 2.37 Å 3 Da -1 and a solvent content of 48.0%.
AB - Succinic semialdehyde dehydrogenase (SSADH) plays a critical role in the metabolism of the inhibitory neurotransmitter γ-aminobutyric acid (GABA) and catalyzes the NAD(P) +-coupled oxidation of succinic semialdehyde (SSA) to succinic acid (SA). SSADH from Streptococcus pyogenes has been purified and crystallized as the apoenzyme and in a complex with NAD +. The crystals of native and NAD +-complexed SSADH diffracted to resolutions of 1.6 and 1.7 Å, respectively, using a synchrotron-radiation source. Both crystals belonged to the orthorhombic space group P2 12 12 1, with unit-cell parameters a = 93.3, b = 100.3, c = 105.1 Å for the native crystal and a = 93.3, b = 100.3, c = 105.0 Å for the complex crystal. Preliminary molecular replacement confirmed the presence of one dimer in both crystals, corresponding to a Matthews coefficient (V M) of 2.37 Å 3 Da -1 and a solvent content of 48.0%.
KW - GabD
KW - NAD
KW - Streptococcus pyogenes
KW - succinic semialdehyde dehydrogenase
UR - http://www.scopus.com/inward/record.url?scp=84858959719&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84858959719&partnerID=8YFLogxK
U2 - 10.1107/S1744309111052055
DO - 10.1107/S1744309111052055
M3 - Article
C2 - 22442224
AN - SCOPUS:84858959719
VL - 68
SP - 288
EP - 291
JO - Acta Crystallographica Section F:Structural Biology Communications
JF - Acta Crystallographica Section F:Structural Biology Communications
SN - 1744-3091
IS - 3
ER -